Substance P in flush tears and Schirmer strips of healthy participants

Markoulli, Maria, Gokhale, Moneisha and You, Jingjing 2017, Substance P in flush tears and Schirmer strips of healthy participants, Optometry and vision science, vol. 94, no. 4, pp. 527-533, doi: 10.1097/OPX.0000000000001040.

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Title Substance P in flush tears and Schirmer strips of healthy participants
Author(s) Markoulli, Maria
Gokhale, Moneisha
You, Jingjing
Journal name Optometry and vision science
Volume number 94
Issue number 4
Start page 527
End page 533
Total pages 7
Publisher Lippincott Williams & Wilkins
Place of publication Philadelphia, Pa.
Publication date 2017-04
ISSN 1538-9235
Keyword(s) Science & Technology
Life Sciences & Biomedicine
human tear film
tear collection
flush tears
Substance P
Summary PURPOSE: To determine the repeatability of the flush tear collection technique and the Schirmer strip for Substance P tear analysis. METHODS: The tears of 10 healthy non-contact-lens wearers were collected via Schirmer strip and microcapillary following instillation of either 20 μL (F-20) or 60 μL (F-60) of saline. Each technique was conducted on two occasions and in a randomized order. Total protein content (TPC) and Substance P concentrations were determined. The overall protein separation profile of each type of tears was examined using one-dimensional gel electrophoresis (1DGE). RESULTS: Collection rates were significantly faster for the F-60 compared to F-20 (17.3 ± 6.9 μL/min and 11.9 ± 5.3 μL/min, respectively, P < .001), with an average Schirmer strip length of 1.5 ± 2.1 mm/min. The coefficient of repeatability between days and eyes was greatest for the Schirmer strip, with eyes and days being significantly different (P = .03 and P = .03, respectively) for Schirmer strip Substance P. TPC was 3.8 ± 2.6 mg/mL, 3.3 ± 1.8 mg/mL, and 3.6 ± 3.0 mg/mL for F-20, F-60, and Schirmer strip techniques, respectively, with no significant difference between techniques (P = .85). Substance P concentration was 13.1 ± 14.8 ng/mL, 9.1 ± 6.1 ng/mL, and 14.9 ± 10.6 ng/mL for F-20, F-60, and Schirmer strip tears, respectively, with no significant difference between techniques (P = .57). 1DGE profile showed similar electrophoresis patterns among F-20, F-60, and basal tears. CONCLUSIONS: The F-60 method allows faster collection than F-20, but the latter results in better repeatability than both the F-60 and Schirmer sampling techniques. All three techniques return the same concentrations of TPC and Substance P. This indicates that tear collection using the F-20 may be more appropriate when conducting comparative analysis, whereas the F-60 may be more appropriate when more volume is required.
Language eng
DOI 10.1097/OPX.0000000000001040
Field of Research 111303 Vision Science
Socio Economic Objective 920107 Hearing
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, American Academy of Optometry
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Document type: Journal Article
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