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Effects of food components that activate TRPA1 receptors on mucosal ion transport in the Mouse intestine

Fothergill, Linda J., Callaghan, Brid, Rivera, Leni R., Lieu, TinaMarie, Poole, Daniel P., Cho, Hyun-Jung, Bravo, David M. and Furness, John B. 2016, Effects of food components that activate TRPA1 receptors on mucosal ion transport in the Mouse intestine, Nutrients, vol. 8, no. 10, pp. 1-11, doi: 10.3390/nu8100623.

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Title Effects of food components that activate TRPA1 receptors on mucosal ion transport in the Mouse intestine
Author(s) Fothergill, Linda J.
Callaghan, Brid
Rivera, Leni R.
Lieu, TinaMarie
Poole, Daniel P.
Cho, Hyun-Jung
Bravo, David M.
Furness, John B.
Journal name Nutrients
Volume number 8
Issue number 10
Start page 1
End page 11
Total pages 11
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2016-10-10
ISSN 2072-6643
Summary TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate TRPA1 receptors (phytonutrients) include allyl isothiocyanate, cinnamaldehyde and linalool, but these may also act at other receptors. Cells lining the intestinal mucosa are immunoreactive for TRPA1 and Trpa1 mRNA occurs in mucosal extracts, suggesting that the TRPA1 receptor is the target for these agonists. However, in situ hybridisation reveals Trpa1 expression in 5-HT containing enteroendocrine cells, not enterocytes. TRPA1 agonists evoke mucosal secretion, which may be indirect (through release of 5-HT) or direct by activation of enterocytes. We investigated effects of the phytonutrients on transmucosal ion currents in mouse duodenum and colon, and the specificity of the phytonutrients in cells transfected with Trpa1, and in Trpa1-deficient mice. The phytonutrients increased currents in the duodenum with the relativepotencies: allyl isothiocyanate (AITC) > cinnamaldehyde > linalool (0.1 to 300 µM). The rank order was similar in the colon, but linalool was ineffective. Responses to AITC were reduced by the TRPA1 antagonist HC-030031 (100 µM), and were greatly diminished in Trpa1-/- duodenum and colon. Responses were not reduced by tetrodotoxin, 5-HT receptor antagonists, or atropine, but inhibition of prostaglandin synthesis reduced responses. Thus, functional TRPA1 channels are expressed by enterocytes of the duodenum and colon. Activation of enterocyte TRPA1 by food components has the potential to facilitate nutrient absorption.
Language eng
DOI 10.3390/nu8100623
Field of Research 111102 Dietetics and Nutrigenomics
1111 Nutrition And Dietetics
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, by the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30091659

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.