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No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats

Hong, Yet Hoi, Betik, Andrew C., Premilovac, Dino, Dwyer, Renee M., Keske, Michelle A., Rattigan, Stephen and McConell, Glen K. 2015, No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats, American journal of physiology: regulatory, integrative and comparative physiology, vol. 308, no. 10, pp. R862-R871, doi: 10.1152/ajpregu.00412.2014.

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Title No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats
Author(s) Hong, Yet Hoi
Betik, Andrew C.
Premilovac, Dino
Dwyer, Renee M.
Keske, Michelle A.ORCID iD for Keske, Michelle A. orcid.org/0000-0003-4214-7628
Rattigan, Stephen
McConell, Glen K.
Journal name American journal of physiology: regulatory, integrative and comparative physiology
Volume number 308
Issue number 10
Start page R862
End page R871
Total pages 10
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2015-05-15
ISSN 1522-1490
Keyword(s) Type 2 diabetes
capillary recruitment
contrast-enhanced ultrasound
femoral blood flow
nitric oxide synthase activity
Animals
Biological Transport
Diabetes Mellitus, Experimental
Diabetes Mellitus, Type 2
Diet, High-Fat
Enzyme Inhibitors
Glucose
Hindlimb
Muscle Contraction
Muscle, Skeletal
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Rats
Rats, Sprague-Dawley
Science & Technology
Life Sciences & Biomedicine
Physiology
Summary Nitric oxide (NO) has been shown to be involved in skeletal muscle glucose uptake during contraction/exercise, especially in individuals with Type 2 diabetes (T2D). To examine the potential mechanisms, we examined the effect of local NO synthase (NOS) inhibition on muscle glucose uptake and muscle capillary blood flow during contraction in healthy and T2D rats. T2D was induced in Sprague-Dawley rats using a combined high-fat diet (23% fat wt/wt for 4 wk) and low-dose streptozotocin injections (35 mg/kg). Anesthetized animals had one hindlimb stimulated to contract in situ for 30 min (2 Hz, 0.1 ms, 35 V) with the contralateral hindlimb rested. After 10 min, the NOS inhibitor, N(G)-nitro-l-arginine methyl ester (l-NAME; 5 μM) or saline was continuously infused into the femoral artery of the contracting hindlimb until the end of contraction. Surprisingly, there was no increase in skeletal muscle NOS activity during contraction in either group. Local NOS inhibition had no effect on systemic blood pressure or muscle contraction force, but it did cause a significant attenuation of the increase in femoral artery blood flow in control and T2D rats. However, NOS inhibition did not attenuate the increase in muscle capillary recruitment during contraction in these rats. Muscle glucose uptake during contraction was significantly higher in T2D rats compared with controls but, unlike our previous findings in hooded Wistar rats, NOS inhibition had no effect on glucose uptake during contraction. In conclusion, NOS inhibition did not affect muscle glucose uptake during contraction in control or T2D Sprague-Dawley rats, and this may have been because there was no increase in NOS activity during contraction.
Language eng
DOI 10.1152/ajpregu.00412.2014
Field of Research 110399 Clinical Sciences not elsewhere classified
110306 Endocrinology
06 Biological Sciences
11 Medical And Health Sciences
Socio Economic Objective 920104 Diabetes
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID NHMRC
Copyright notice ©2015, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30092192

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