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Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats

St-Pierre, P., Keith, L. J., Richards, S. M., Rattigan, S. and Keske, M. A. 2012, Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats, Diabetes, obesity and metabolism, vol. 14, no. 8, pp. 753-761, doi: 10.1111/j.1463-1326.2012.01598.x.

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Title Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats
Author(s) St-Pierre, P.
Keith, L. J.
Richards, S. M.
Rattigan, S.
Keske, M. A.ORCID iD for Keske, M. A. orcid.org/0000-0003-4214-7628
Journal name Diabetes, obesity and metabolism
Volume number 14
Issue number 8
Start page 753
End page 761
Total pages 9
Publisher Wiley
Place of publication Chichester, Eng.
Publication date 2012-08
ISSN 1462-8902
1463-1326
Keyword(s) dietary fat
insulin resistance
microvascular perfusion
muscle glucose uptake
Animals
Blood Glucose
Central Nervous System Stimulants
Diet, High-Fat
Electric Stimulation
Femoral Artery
Hindlimb
Male
Muscle Contraction
Muscle, Skeletal
Rats
Rats, Sprague-Dawley
Rats, Wistar
Regional Blood Flow
Xanthines
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
MEDIATED CAPILLARY RECRUITMENT
INDUCED INSULIN-RESISTANCE
HUMAN FOREARM MUSCLE
UPTAKE IN-VIVO
SKELETAL-MUSCLE
GLUCOSE-UPTAKE
ACUTE EXERCISE
MIXED MEAL
SENSITIVITY
INHIBITION
Summary AIM: Exercise and insulin each increase microvascular blood flow and enhance glucose disposal in skeletal muscle. We have reported that insulin-mediated microvascular recruitment in a diet-induced model of insulin resistance (high-fat feeding for 4 weeks) is markedly impaired; however, the effect of muscle contraction in this model has not been previously explored.

METHODS: We fed rats either normal (ND, 10% calories from fat) or high-fat (HFD, 60% calories from fat) diets ad libitum for 4-8 weeks. Animals were then anaesthetized and one hindlimb electrically stimulated to contract at 0.05, 0.1 and 2 Hz (field stimulation, 30 V, 0.1 ms duration) in 15 min stepwise increments. Femoral artery blood flow (Transonic flow probe), muscle microvascular blood flow (hindleg metabolism of 1-methylxanthine and contrast-enhanced ultrasound) and muscle glucose disposal (uptake of radiolabelled 2-deoxy-ᴅ-glucose and hindleg glucose disappearance) were measured.

RESULTS: Both ND and HFD rats received the same voltage across the leg and consequently developed the same muscle tension. Femoral artery blood flow in the contracting leg increased during 2 Hz contraction, but not during the lower frequencies and these effects were similar between ND and HFD rats. Muscle microvascular blood flow significantly increased in a contraction frequency-dependent manner, and preceded increases in total limb blood flow and these effects were similar between ND and HFD rats. Muscle glucose disposal was markedly elevated during 2 Hz contraction and was comparable between ND and HFD rats.

CONCLUSION: Contraction-mediated muscle microvascular recruitment and glucose uptake are not impaired in the HFD insulin resistant rat.
Language eng
DOI 10.1111/j.1463-1326.2012.01598.x
Field of Research 110602 Exercise Physiology
110306 Endocrinology
111103 Nutritional Physiology
1103 Clinical Sciences
Socio Economic Objective 920104 Diabetes
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, Blackwell
Persistent URL http://hdl.handle.net/10536/DRO/DU:30092220

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