You are not logged in.
Openly accessible

Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate

Counihan, Natalie A., Chisholm, Scott A., Bullen, Hayley E., Srivastava, Anubhav, Sanders, Paul R., Jonsdottir, Thorey K., Weiss, Greta E., Ghosh, Sreejoyee, Crabb, Brendan S., Creek, Darren J., Gilson, Paul R. and de Koning-Ward, Tania F. 2017, Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate, eLife, vol. 6, pp. 1-31, doi: 10.7554/eLife.23217.

Attached Files
Name Description MIMEType Size Downloads
counihan-plasmodiumfalciparum-2017.pdf Published version application/pdf 3.62MB 2

Title Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate
Formatted title Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate
Author(s) Counihan, Natalie A.
Chisholm, Scott A.
Bullen, Hayley E.
Srivastava, Anubhav
Sanders, Paul R.
Jonsdottir, Thorey K.
Weiss, Greta E.
Ghosh, Sreejoyee
Crabb, Brendan S.
Creek, Darren J.
Gilson, Paul R.
de Koning-Ward, Tania F.ORCID iD for de Koning-Ward, Tania F. orcid.org/0000-0001-5810-8063
Journal name eLife
Volume number 6
Article ID e23217
Start page 1
End page 31
Total pages 31
Publisher eLife Sciences
Place of publication Cambridge, Eng.
Publication date 2017-03-02
ISSN 2050-084X
Keyword(s) inducible expression
P. berghei
cell biology
host cell remodeling
infectious disease
microbiology
rhoptry
Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
RED-BLOOD-CELLS
HUMAN MALARIA PARASITE
CLAG MULTIGENE FAMILY
RHOPTRY PROTEIN
PTEX COMPONENT
INFECTED ERYTHROCYTES
FUNCTIONAL-ANALYSIS
MEROZOITE INVASION
MAURERS CLEFTS
MEMBRANE
Summary Plasmodium falciparum parasites, the causative agents of malaria, modify their host erythrocyte to render them permeable to supplementary nutrient uptake from the plasma and for removal of toxic waste. Here we investigate the contribution of the rhoptry protein RhopH2, in the formation of new permeability pathways (NPPs) in Plasmodium-infected erythrocytes. We show RhopH2 interacts with RhopH1, RhopH3, the erythrocyte cytoskeleton and exported proteins involved in host cell remodeling. Knockdown of RhopH2 expression in cycle one leads to a depletion of essential vitamins and cofactors and decreased de novo synthesis of pyrimidines in cycle two. There is also a significant impact on parasite growth, replication and transition into cycle three. The uptake of solutes that use NPPs to enter erythrocytes is also reduced upon RhopH2 knockdown. These findings provide direct genetic support for the contribution of the RhopH complex in NPP activity and highlight the importance of NPPs to parasite survival.
Language eng
DOI 10.7554/eLife.23217
Field of Research 110799 Immunology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30092958

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 13 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Tue, 04 Apr 2017, 15:21:12 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.