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Hypothiocyanite produced by human and rat respiratory epithelial cells inactivates extracellular H1N2 influenza A virus

Gingerich, Aaron, Pang, Lan, Hanson, Jarod, Dlugolenski, Daniel, Streich, Rebecca, Lafontaine, Eric R., Nagy, Tamas, Tripp, Ralph A. and Rada, Balazs 2016, Hypothiocyanite produced by human and rat respiratory epithelial cells inactivates extracellular H1N2 influenza A virus, Inflammation research, vol. 65, no. 1, pp. 71-80, doi: 10.1007/s00011-015-0892-z.

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Title Hypothiocyanite produced by human and rat respiratory epithelial cells inactivates extracellular H1N2 influenza A virus
Author(s) Gingerich, Aaron
Pang, Lan
Hanson, Jarod
Dlugolenski, Daniel
Streich, Rebecca
Lafontaine, Eric R.
Nagy, Tamas
Tripp, Ralph A.
Rada, Balazs
Journal name Inflammation research
Volume number 65
Issue number 1
Start page 71
End page 80
Total pages 10
Publisher Springer
Place of publication Basel, Switzerland
Publication date 2016-01
ISSN 1023-3830
1420-908X
Keyword(s) dual oxidase
hypothiocyanite
influenza
airway epithelium
hydrogen peroxide
Animals
Dogs
Epithelial Cells
Humans
Influenza A Virus, H1N2 Subtype
Lactoperoxidase
Madin Darby Canine Kidney Cells
Male
Mucins
Primary Cell Culture
Rats
Rats, Sprague-Dawley
Respiratory Mucosa
Thiocyanates
Summary OBJECTIVE AND DESIGN: Our aim was to study whether an extracellular, oxidative antimicrobial mechanism inherent to tracheal epithelial cells is capable of inactivating influenza H1N2 virus.

MATERIAL OR SUBJECTS: Epithelial cells were isolated from tracheas of male Sprague-Dawley rats. Both primary human and rat tracheobronchial epithelial cells were differentiated in air-liquid interface cultures.

TREATMENT: A/swine/Illinois/02860/09 (swH1N2) influenza A virions were added to the apical side of airway cells for 1 h in the presence or absence of lactoperoxidase or thiocyanate.

METHODS: Characterization of rat epithelial cells (morphology, Duox expression) occurred via western blotting, PCR, hydrogen peroxide production measurement and histology. The number of viable virions was determined by plaque assays. Statistical difference of the results was analyzed by ANOVA and Tukey's test.

RESULTS: Our data show that rat tracheobronchial epithelial cells develop a differentiated, polarized monolayer with high transepithelial electrical resistance, mucin production and expression of dual oxidases. Influenza A virions are inactivated by human and rat epithelial cells via a dual oxidase-, lactoperoxidase- and thiocyanate-dependent mechanism.

CONCLUSIONS: Differentiated air-liquid interface cultures of rat tracheal epithelial cells provide a novel model to study airway epithelium-influenza interactions. The dual oxidase/lactoperoxidase/thiocyanate extracellular oxidative system producing hypothiocyanite is a fast and potent anti-influenza mechanism inactivating H1N2 viruses prior to infection of the epithelium.
Language eng
DOI 10.1007/s00011-015-0892-z
Field of Research 110399 Clinical Sciences not elsewhere classified
1103 Clinical Sciences
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30092982

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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