You are not logged in.

Phaleria macrocarpa (Boerl.) fruit induce G₀/G₁ and G₂/M cell cycle arrest and apoptosis through mitochondria-mediated pathway in MDA-MB-231 human breast cancer cell

Kavitha, Nowroji, Ein Oon, Chern, Chen, Yeng, Kanwar, Jagat K. and Sasidharan, Sreenivasan 2017, Phaleria macrocarpa (Boerl.) fruit induce G₀/G₁ and G₂/M cell cycle arrest and apoptosis through mitochondria-mediated pathway in MDA-MB-231 human breast cancer cell, Journal ethnopharmacology, vol. 201, pp. 42-55, doi: 10.1016/j.jep.2017.02.041.

Attached Files
Name Description MIMEType Size Downloads

Title Phaleria macrocarpa (Boerl.) fruit induce G₀/G₁ and G₂/M cell cycle arrest and apoptosis through mitochondria-mediated pathway in MDA-MB-231 human breast cancer cell
Author(s) Kavitha, Nowroji
Ein Oon, Chern
Chen, Yeng
Kanwar, Jagat K.
Sasidharan, Sreenivasan
Journal name Journal ethnopharmacology
Volume number 201
Start page 42
End page 55
Total pages 14
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2017-04-06
ISSN 0378-8741
1872-7573
Keyword(s) apoptosis
caspase, p53, cytochrome c
cells cycle arrest
THERAPY
Summary ETHNOPHARMACOLOGICAL RELEVANCE: Phaleria macrocarpa (Scheff) Boerl, is a well-known folk medicinal plant in Indonesia. Traditionally, P. macrocarpa has been used to control cancer, impotency, hemorrhoids, diabetes mellitus, allergies, liver and hearth disease, kidney disorders, blood diseases, acne, stroke, migraine, and various skin diseases. AIM OF THE STUDY: The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) and the underlying molecular mechanism of cell death. MATERIALS AND METHODS: MDA-MB-231 cells were incubated with PMEAF for 24h. Cell cycle and viability were examined using flow cytometry analysis. Apoptosis was determined using the Annexin V assay and also by fluorescence microscopy. Apoptosis protein profiling was detected by RayBio® Human Apoptosis Array. RESULTS: The AO/PI staining and flow cytometric analysis of MDA-MB-231 cells treated with PMEAF were showed apoptotic cell death. The cell cycle analysis by flow cytometry analysis revealed that the accumulation of PMEAF treated MDA-MB-231 cells in G0/G1 and G2/M-phase of the cell cycle. Moreover, the PMEAF exert cytotoxicity by increased the ROS production in MDA-MB-231 cells consistently stimulated the loss of mitochondrial membrane potential (∆Ψm) and induced apoptosis cell death by activation of numerous signalling proteins. The results from apoptosis protein profiling array evidenced that PMEAF stimulated the expression of 9 pro-apoptotic proteins (Bax, Bid, caspase 3, caspase 8, cytochrome c, p21, p27, p53 and SMAC) and suppressed the 4 anti-apoptotic proteins (Bcl-2, Bcl-w, XIAP and survivin) in MDA-MB-231 cells. CONCLUSION: The results indicated that PMEAF treatment induced apoptosis in MDA-MB-231 cells through intrinsic mitochondrial related pathway with the participation of pro and anti-apoptotic proteins, caspases, G0/G1 and G2/M-phases cell cycle arrest by p53-mediated mechanism.
Language eng
DOI 10.1016/j.jep.2017.02.041
Field of Research 111299 Oncology and Carcinogenesis not elsewhere classified
0607 Plant Biology
1115 Pharmacology And Pharmaceutical Sciences
1104 Complementary And Alternative Medicine
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30093178

Document type: Journal Article
Collection: School of Medicine
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 12 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Wed, 05 Apr 2017, 10:43:36 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.