In situ morphological assessment of apoptosis induced by Phaleria macrocarpa (Boerl.) fruit ethyl acetate fraction (PMEAF) in MDA-MB-231 cells by microscopy observation

Kavitha, Nowroji, Chen, Yeng, Kanwar, Jagat R. and Sasidharan, Sreenivasan 2017, In situ morphological assessment of apoptosis induced by Phaleria macrocarpa (Boerl.) fruit ethyl acetate fraction (PMEAF) in MDA-MB-231 cells by microscopy observation, Biomedicine and pharmacotherapy, vol. 87, pp. 609-620, doi: 10.1016/j.biopha.2016.12.127.

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Title In situ morphological assessment of apoptosis induced by Phaleria macrocarpa (Boerl.) fruit ethyl acetate fraction (PMEAF) in MDA-MB-231 cells by microscopy observation
Author(s) Kavitha, Nowroji
Chen, Yeng
Kanwar, Jagat R.ORCID iD for Kanwar, Jagat R. orcid.org/0000-0003-3728-9568
Sasidharan, Sreenivasan
Journal name Biomedicine and pharmacotherapy
Volume number 87
Start page 609
End page 620
Total pages 12
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2017-03
ISSN 1950-6007
Keyword(s) Apoptosis
CyQUANT assay
Electron microscopy
Holography image
MDA-MB-231cells
Medicinal plant
Acetates
Antineoplastic Agents
Cell Line, Tumor
Cell Proliferation
Fruit
Humans
Microscopy
Plant Extracts
Plants, Medicinal
Thymelaeaceae
Summary Phaleria macrocarpa (Boerl.) is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases. The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. The cytotoxicity of PMEAF treated MDA-MB-231 cells was determined through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay and CyQuant Cell Proliferation Assay after 24h of treatment. Both results were indicated that the PMEAF is a potential anticancer agent with the average IC50 values of 18.10μg/mL by inhibiting the MDA-MB-231 cell proliferation. Various conventional and modern microscopy techniques such as light microscopy, holographic microscopy, transmission (TEM) and scanning (SEM) electron microscope were used for the observation of morphological changes in PMEAF treated MDA-MB-231cells for 24h. The characteristic of apoptotic cell death includes cell shrinkage, membrane blebs, chromatin condensation and the formation of apoptotic bodies were observed. PMEAF might be the best candidate for developing more potent anticancer drugs or chemo-preventive supplements.
Language eng
DOI 10.1016/j.biopha.2016.12.127
Field of Research 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
1115 Pharmacology And Pharmaceutical Sciences
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017 Elsevier Masson SAS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30093179

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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