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Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

Singh, Nisha, Mathur, Anshu S, Tuli, Deepak K, Gupta, Ravi P, Barrow, Colin J and Puri, Munish 2017, Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring, Biotechnology for biofuels, vol. 10, pp. 1-18, doi: 10.1186/s13068-017-0756-6.

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Title Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring
Author(s) Singh, Nisha
Mathur, Anshu S
Tuli, Deepak K
Gupta, Ravi P
Barrow, Colin JORCID iD for Barrow, Colin J orcid.org/0000-0002-2153-7267
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Biotechnology for biofuels
Volume number 10
Article ID 73
Start page 1
End page 18
Total pages 18
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2017
ISSN 1754-6834
Keyword(s) Cellulosic ethanol
Co-culture fermentation
Dilute acid pretreatment
Rice straw
Science & Technology
Life Sciences & Biomedicine
Technology
Biotechnology & Applied Microbiology
Energy & Fuels
Summary Background
Cellulose-degrading thermophilic anaerobic bacterium as a suitable host for consolidated bioprocessing (CBP) has been proposed as an economically suited platform for the production of second-generation biofuels. To recognize the overall objective of CBP, fermentation using co-culture of different cellulolytic and sugar-fermenting thermophilic anaerobic bacteria has been widely studied as an approach to achieving improved ethanol production. We assessed monoculture and co-culture fermentation of novel thermophilic anaerobic bacterium for ethanol production from real substrates under controlled conditions.

Results

In this study, Clostridium sp. DBT-IOC-C19, a cellulose-degrading thermophilic anaerobic bacterium, was isolated from the cellulolytic enrichment cultures obtained from a Himalayan hot spring. Strain DBT-IOC-C19 exhibited a broad substrate spectrum and presented single-step conversion of various cellulosic and hemicellulosic substrates to ethanol, acetate, and lactate with ethanol being the major fermentation product. Additionally, the effect of varying cellulose concentrations on the fermentation performance of the strain was studied, indicating a maximum cellulose utilization ability of 10 g L−1 cellulose. Avicel degradation kinetics of the strain DBT-IOC-C19 displayed 94.6% degradation at 5 g L−1 and 82.74% degradation at 10 g L−1 avicel concentration within 96 h of fermentation. In a comparative study with Clostridium thermocellum DSM 1313, the ethanol and total product concentrations were higher by the newly isolated strain on pretreated rice straw at an equivalent substrate loading. Three different co-culture combinations were used on various substrates that presented two-fold yield improvement than the monoculture during batch fermentation.

Conclusions
This study demonstrated the direct fermentation ability of the novel thermophilic anaerobic bacteria on various cellulosic and hemicellulosic substrates into ethanol without the aid of any exogenous enzymes, representing CBP-based fermentation approach. Here, the broad substrate utilization spectrum of isolated cellulolytic thermophilic anaerobic bacterium was shown to be of potential utility. We demonstrated that the co-culture strategy involving novel strains is efficient in improving ethanol production from real substrate.
Language eng
DOI 10.1186/s13068-017-0756-6
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
030199 Analytical Chemistry not elsewhere classified
0904 Chemical Engineering
1003 Industrial Biotechnology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30093586

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.