The skin barrier function gene SPINK5 is associated with challenge-proven IgE-mediated food allergy in infants

Ashley, S.E., Tan, H-T.T., Vuillermin, P., Dharmage, S.C., Tang, M.L.K., Koplin, J., Gurrin, L.C., Lowe, A., Lodge, C., Ponsonby, A-L., Molloy, J., Martin, P., Matheson, M.C., Saffery, R., Allen, K.J., Ellis, J.A., Martino, D. and HealthNuts team 2017, The skin barrier function gene SPINK5 is associated with challenge-proven IgE-mediated food allergy in infants, Allergy, pp. 1-9, doi: 10.1111/all.13143.

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Title The skin barrier function gene SPINK5 is associated with challenge-proven IgE-mediated food allergy in infants
Author(s) Ashley, S.E.
Tan, H-T.T.
Vuillermin, P.ORCID iD for Vuillermin, P.
Dharmage, S.C.
Tang, M.L.K.
Koplin, J.
Gurrin, L.C.
Lowe, A.
Lodge, C.
Ponsonby, A-L.
Molloy, J.
Martin, P.
Matheson, M.C.
Saffery, R.
Allen, K.J.
Ellis, J.A.
Martino, D.
HealthNuts team
Journal name Allergy
Start page 1
End page 9
Total pages 9
Publisher Wiley-Blackwell
Place of publication Chichester, Eng.
Publication date 2017-02-18
ISSN 1398-9995
Keyword(s) serine peptidase inhibitor Kazal type 5
food allergy
lymphoepithelial Kazal-type-related inhibitor
skin barrier
skin barrier function
HealthNuts team
Barwon Infant Study, the Melbourne Atopy Cohort study, the Peanut Allergen Threshold Study and the Probiotic and Peanut Oral ImmunoTherapy study
Summary BACKGROUND: A defective skin barrier is hypothesized to be an important route of sensitization to dietary antigens and may lead to food allergy in some children. Missense mutations in the serine peptidase inhibitor Kazal type 5 (SPINK5) skin barrier gene have previously been associated with allergic conditions. OBJECTIVE: To determine whether genetic variants in and around SPINK5 are associated with IgE-mediated food allergy. METHOD: We genotyped 71 "tag" single nucleotide polymorphisms (tag-SNPs) within a region spanning ~263 kb including SPINK5 (~61 kb) in n=722 (n=367 food-allergic, n=199 food-sensitized-tolerant and n=156 non-food-allergic controls) 12-month-old infants (discovery sample) phenotyped for food allergy with the gold standard oral food challenge. Transepidermal water loss (TEWL) measures were collected at 12 months from a subset (n=150) of these individuals. SNPs were tested for association with food allergy using the Cochran-Mantel-Haenszel test adjusting for ancestry strata. Association analyses were replicated in an independent sample group derived from four paediatric cohorts, total n=533 (n=203 food-allergic, n=330 non-food-allergic), mean age 2.5 years, with food allergy defined by either clinical history of reactivity, 95% positive predictive value (PPV) or challenge, corrected for ancestry by principal components. RESULTS: SPINK5 variant rs9325071 (A⟶G) was associated with challenge-proven food allergy in the discovery sample (P=.001, OR=2.95, CI=1.49-5.83). This association was further supported by replication (P=.007, OR=1.58, CI=1.13-2.20) and by meta-analysis (P=.0004, OR=1.65). Variant rs9325071 is associated with decreased SPINK5 gene expression in the skin in publicly available genotype-tissue expression data, and we generated preliminary evidence for association of this SNP with elevated TEWL also. CONCLUSIONS: We report, for the first time, association between SPINK5 variant rs9325071 and challenge-proven IgE-mediated food allergy.
Notes In Press
Language eng
DOI 10.1111/all.13143
Field of Research 110799 Immunology not elsewhere classified
1107 Immunology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, EAACI & Wiley-Blackwell
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Collections: Faculty of Health
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