You are not logged in.

Glucose-loading reduces bone remodeling in women and osteoblast function in vitro

Levinger, Itamar, Seeman, Ego, Jerums, George, McConell, Glenn K., Rybchyn, Mark S., Cassar, Samantha, Byrnes, Elizabeth, Selig, Steve, Mason, Rebecca S., Ebeling, Peter R. and Brennan-Speranza, Tara C. 2016, Glucose-loading reduces bone remodeling in women and osteoblast function in vitro, Physiological reports, vol. 4, no. 3, Article no. e12700, pp. 1-10, doi: 10.14814/phy2.12700.

Attached Files
Name Description MIMEType Size Downloads

Title Glucose-loading reduces bone remodeling in women and osteoblast function in vitro
Author(s) Levinger, Itamar
Seeman, Ego
Jerums, George
McConell, Glenn K.
Rybchyn, Mark S.
Cassar, Samantha
Byrnes, Elizabeth
Selig, Steve
Mason, Rebecca S.
Ebeling, Peter R.
Brennan-Speranza, Tara C.
Journal name Physiological reports
Volume number 4
Issue number 3
Season Article no. e12700
Start page 1
End page 10
Total pages 10
Publisher Wiley
Place of publication Chichester, Eng.
Publication date 2016-02-04
ISSN 2051-817X
Keyword(s) bone remodeling
glycemic control
in vitro
in vivo
osteoblasts function
Blood Glucose
Cells, Cultured
Cross-Over Studies
Glucose Tolerance Test
Middle Aged
Summary Aging is associated with a reduction in osteoblast life span and the volume of bone formed by each basic multicellular unit. Each time bone is resorbed, less is deposited producing microstructural deterioration. Aging is also associated with insulin resistance and hyperglycemia, either of which may cause, or be the result of, a decline in undercarboxylated osteocalcin (ucOC), a protein produced by osteoblasts that increases insulin sensitivity. We examined whether glucose-loading reduces bone remodeling and ucOC in vivo and osteoblast function in vitro, and so compromises bone formation. We administered an oral glucose tolerance test (OGTT) to 18 pre and postmenopausal, nondiabetic women at rest and following exercise and measured serum levels of bone remodeling markers (BRMs) and ucOC. We also assessed whether increasing glucose concentrations with or without insulin reduced survival and activity of cultured human osteoblasts. Glucose-loading at rest and following exercise reduced BRMs in pre and postmenopausal women and reduced ucOC in postmenopausal women. Higher glucose correlated negatively, whereas insulin correlated positively, with baseline BRMs and ucOC. The increase in serum glucose following resting OGTT was associated with the reduction in bone formation markers. D-glucose (>10 mmol L-1) increased osteoblast apoptosis, reduced cell activity and osteocalcin expression compared with 5 mmol L-1. Insulin had a protective effect on these parameters. Collagen expression in vitro was not affected in this time course. In conclusion, glucose exposure reduces BRMs in women and exercise failed to attenuate this suppression effect. The suppressive effect of glucose on BRMs may be due to impaired osteoblast work and longevity. Whether glucose influences material composition and microstructure remains to be determined.
Language eng
DOI 10.14814/phy2.12700
Field of Research 110399 Clinical Sciences not elsewhere classified
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Persistent URL

Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 9 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 8 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Fri, 07 Apr 2017, 11:30:37 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact