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Relaxin-3 receptor (RXFP3) signalling mediates stress-related alcohol preference in mice

Walker, Andrew W., Smith, Craig M., Chua, Berenice E., Krstew, Elena V., Zhang, Cary, Gundlach, Andrew L. and Lawrence, Andrew J. 2015, Relaxin-3 receptor (RXFP3) signalling mediates stress-related alcohol preference in mice, PLoS One, vol. 10, no. 4, pp. 1-71, doi: 10.1371/journal.pone.0122504.

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Title Relaxin-3 receptor (RXFP3) signalling mediates stress-related alcohol preference in mice
Author(s) Walker, Andrew W.
Smith, Craig M.ORCID iD for Smith, Craig M. orcid.org/0000-0002-2894-2433
Chua, Berenice E.
Krstew, Elena V.
Zhang, Cary
Gundlach, Andrew L.
Lawrence, Andrew J.
Journal name PLoS One
Volume number 10
Issue number 4
Start page 1
End page 71
Total pages 17
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2015-04-07
ISSN 1932-6203
Keyword(s) Alcohol Dehydrogenase
Alcohol Drinking
Aldehyde Dehydrogenase
Animals
Ethanol
Feeding Behavior
Liver
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Rats
Receptors, G-Protein-Coupled
Saccharin
Signal Transduction
Stress, Physiological
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
CORTICOTROPIN-RELEASING-FACTOR
CHRONIC SWIM STRESS
LONG-EVANS RATS
NUCLEUS-INCERTUS
STRIA TERMINALIS
MESSENGER-RNA
BED NUCLEUS
MATERNAL SEPARATION
MACACA-FASCICULARIS
ETHANOL-CONSUMPTION
Summary Stressful life events are causally linked with alcohol use disorders (AUDs), providing support for a hypothesis that alcohol consumption is aimed at stress reduction. We have previously shown that expression of relaxin-3 mRNA in rat brain correlates with alcohol intake and that central antagonism of relaxin-3 receptors (RXFP3) prevents stress-induced reinstatement of alcohol-seeking. Therefore the objectives of these studies were to investigate the impact of Rxfp3 gene deletion in C57BL/6J mice on baseline and stress-related alcohol consumption. Male wild-type (WT) and Rxfp3 knockout (KO) (C57/B6JRXFP3TM1/DGen) littermate mice were tested for baseline saccharin and alcohol consumption and preference over water in a continuous access two-bottle free-choice paradigm. Another cohort of mice was subjected to repeated restraint followed by swim stress to examine stress-related alcohol preference. Hepatic alcohol and aldehyde dehydrogenase activity was assessed in mice following chronic alcohol intake and in naive controls. WT and Rxfp3 KO mice had similar baseline saccharin and alcohol preference, and hepatic alcohol processing. However, Rxfp3 KO mice displayed a stress-induced reduction in alcohol preference that was not observed in WT littermates. Notably, this phenotype, once established, persisted for at least six weeks after cessation of stress exposure. These findings suggest that in mice, relaxin-3/RXFP3 signalling is involved in maintaining high alcohol preference during and after stress, but does not appear to strongly regulate the primary reinforcing effects of alcohol.
Language eng
DOI 10.1371/journal.pone.0122504
Field of Research 110999 Neurosciences not elsewhere classified
MD Multidisciplinary
Socio Economic Objective 970117 Expanding Knowledge in Psychology and Cognitive Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30093780

Document type: Journal Article
Collections: School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.