Isotope dilution hplc-ms/ms for simultaneous quantification of acrylamide and 5-hydroxymethylfurfural (hmf) in thermally processed seafood

Qin, Lei, Zhang, Yu-Ying, Xu, Xian-Bing, Wang, Xu-Song, Liu, Hong-Wei, Zhou, Da-Yong, Zhu, Bei-Wei and Thornton, Megan 2017, Isotope dilution hplc-ms/ms for simultaneous quantification of acrylamide and 5-hydroxymethylfurfural (hmf) in thermally processed seafood, Food Chemistry, vol. 232, pp. 633-638, doi: 10.1016/j.foodchem.2017.04.069.

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Title Isotope dilution hplc-ms/ms for simultaneous quantification of acrylamide and 5-hydroxymethylfurfural (hmf) in thermally processed seafood
Author(s) Qin, Lei
Zhang, Yu-Ying
Xu, Xian-Bing
Wang, Xu-Song
Liu, Hong-Wei
Zhou, Da-Yong
Zhu, Bei-Wei
Thornton, MeganORCID iD for Thornton, Megan orcid.org/0000-0003-0722-0906
Journal name Food Chemistry
Volume number 232
Start page 633
End page 638
Total pages 6
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2017-10
ISSN 0308-8146
Keyword(s) 5-Hydroxymethylfurfural
Acrylamide
HPLC-MS/MS
Isotope dilution
Seafood
Science & Technology
Physical Sciences
Life Sciences & Biomedicine
Chemistry, Applied
Food Science & Technology
Nutrition & Dietetics
Chemistry
SOLID-PHASE EXTRACTION
MASS-SPECTROMETRY
LC-MS/MS
FOODS
QUANTITATION
GLYCIDAMIDE
METABOLITE
BAKING
MODEL
GC
Summary The objective of this study was to develop an accurate, robust and rapid HPLC-MS/MS-based method capable of quantifying acrylamide and 5-hydroxymethylfurfural (HMF) simultaneously. A Phenomenex Synergi Fusion-RP C18 column (50mm×2.5mm, 2μm) was used, and the MS/MS instrument was operated in multiple reaction monitoring mode. The isotope dilution method was used to correct for the matrix effect from samples. This method demonstrated low limits of quantification (2.12ng/mL for acrylamide and 4.86ng/mL for HMF) and excellent linearity (R(2)>0.999). This method also demonstrated excellent quantification accuracy, precision, and recovery (87-110%). Using the method developed, target analytes were quantified in 14 thermally-processed seafood samples. Resulting concentrations ranged from 5.58 to 50.35μg/kg for acrylamide, and from 12.54 to 2276.44μg/kg for HMF. The proposed isotope dilution HPLC-MS/MS method is a valid and rapid technique for simultaneous analysis of acrylamide and HMF in seafood.
Language eng
DOI 10.1016/j.foodchem.2017.04.069
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
MD Multidisciplinary
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017 Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30094573

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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