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A reduction in selenoprotein S amplifies the inflammatory profile of fast-twitch skeletal muscle in the mdx dystrophic mouse

Wright, Craig Robert, Allsopp, Giselle Larissa, Addinsall, Alex Bernard, McRae, Natasha Lee, Andrikopoulos, Sofianos and Stupka, Nicole 2017, A reduction in selenoprotein S amplifies the inflammatory profile of fast-twitch skeletal muscle in the mdx dystrophic mouse, Mediators of inflammation, vol. 2017, pp. 1-12, doi: 10.1155/2017/7043429.

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Title A reduction in selenoprotein S amplifies the inflammatory profile of fast-twitch skeletal muscle in the mdx dystrophic mouse
Author(s) Wright, Craig RobertORCID iD for Wright, Craig Robert orcid.org/0000-0001-7903-3144
Allsopp, Giselle LarissaORCID iD for Allsopp, Giselle Larissa orcid.org/0000-0003-1124-7706
Addinsall, Alex Bernard
McRae, Natasha Lee
Andrikopoulos, Sofianos
Stupka, Nicole
Journal name Mediators of inflammation
Volume number 2017
Article ID 7043429
Start page 1
End page 12
Total pages 12
Publisher Hindawi Publishing Corporation
Place of publication Cairo, Egypt
Publication date 2017
ISSN 1466-1861
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Cell Biology
Immunology
Summary Excessive inflammation is a hallmark of muscle myopathies, including Duchenne muscular dystrophy (DMD). There is interest in characterising novel genes that regulate inflammation due to their potential to modify disease progression. Gene polymorphisms in Selenoprotein S (Seps1) are associated with elevated proinflammatory cytokines, and in vitro SEPS1 is protective against inflammatory stress. Given that SEPS1 is highly expressed in skeletal muscle, we investigated whether the genetic reduction of Seps1 exacerbated inflammation in the mdx mouse. F1 male mdx mice with a heterozygous Seps1 deletion (mdx:Seps1−/+) were generated. The mdx:Seps1−/+ mice had a 50% reduction in SEPS1 protein expression in hindlimb muscles. In the extensor digitorum longus (EDL) muscles, mRNA expression of monocyte chemoattractant protein 1 (Mcp-1) (), macrophage marker F4/80 (), and transforming growth factor-β1 (Tgf-β1) () were increased in mdx:Seps1−/+ mice. This was associated with a reduction in muscle fibre size; however, ex vivo EDL muscle strength and endurance were unaltered. In dystrophic slow twitch soleus muscles, SEPS1 reduction had no effect on the inflammatory profile nor function. In conclusion, the genetic reduction of Seps1 appears to specifically exacerbate the inflammatory profile of fast-twitch muscle fibres, which are typically more vulnerable to degeneration in dystrophy.
Language eng
DOI 10.1155/2017/7043429
Field of Research 1107 Immunology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30099843

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.