Identification of an rsh gene from a Novosphingobium sp. necessary for quorum-sensing signal accumulation

Gan, Han Ming, Buckley, L, Szegedi, E, Hudson, AO and Savka, MA 2009, Identification of an rsh gene from a Novosphingobium sp. necessary for quorum-sensing signal accumulation, Journal of bacteriology, vol. 191, no. 8, pp. 2551-2560, doi: 10.1128/JB.01692-08.

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Title Identification of an rsh gene from a Novosphingobium sp. necessary for quorum-sensing signal accumulation
Author(s) Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Buckley, L
Szegedi, E
Hudson, AO
Savka, MA
Journal name Journal of bacteriology
Volume number 191
Issue number 8
Start page 2551
End page 2560
Total pages 10
Publisher American Society for Microbiology
Place of publication United States
Publication date 2009-04
ISSN 1098-5530
Keyword(s) Acyl-Butyrolactones
Amino Acid Sequence
Bacterial Adhesion
Bacterial Proteins
DNA Transposable Elements
DNA, Bacterial
DNA, Ribosomal
Escherichia coli
Genes, rRNA
Genetic Complementation Test
Molecular Sequence Data
Mutagenesis, Insertional
Phylogeny
Polysaccharides, Bacterial
Quorum Sensing
RNA, Bacterial
RNA, Ribosomal, 16S
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Sphingomonadaceae
Vitis
Science & Technology
Life Sciences & Biomedicine
Microbiology
N-ACYLHOMOSERINE LACTONES
CELL-CELL COMMUNICATION
NEIGHBOR-JOINING METHOD
STRINGENT RESPONSE
AGROBACTERIUM-TUMEFACIENS
PSEUDOMONAS-AERUGINOSA
ESCHERICHIA-COLI
RHIZOBIUM-MELILOTI
ALARMONE PPGPP
CROWN-GALL
Summary The stringent response is a mechanism by which bacteria adapt to environmental stresses and nutritional deficiencies through the synthesis and hydrolysis of (p)ppGpp by RelA/SpoT enzymes. Alphaproteobacteria and plants contain a single Rsh enzyme (named for RelA/SpoT homolog) that is bifunctional. Here we report the identification of a new species of bacteria belonging to the genus Novosphingobium and characterization of an rsh mutation in this plant tumor-associated isolate. Isolate Rr 2-17, from a grapevine crown gall tumor, is a member of the Novosphingobium genus that produces the N-acyl-homoserine lactone (AHL) quorum-sensing (QS) signals. A Tn5 mutant, Hx 699, deficient in AHL production was found to have an insertion in an rsh gene. The Rsh protein showed significant percent sequence identity to Rsh proteins of alphaproteobacteria. The Novosphingobium sp. rsh gene (rsh(Nsp)) complemented the multiple amino acid requirements of the Escherichia coli relA spoT double mutant by restoring the growth on selection media. Besides QS signal production, the rsh mutation also affects soluble polysaccharide production and cell aggregation. Genetic complementation of the Hx 699 mutant with the rsh(Nsp) gene restored these phenotypes. This is the first discovery of a functional rsh gene in a member of the Novosphingobium genus.
Language eng
DOI 10.1128/JB.01692-08
Field of Research 06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Persistent URL http://hdl.handle.net/10536/DRO/DU:30101893

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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