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Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia

Daniel, Diane S., Lee, Sui M., Gan, Han M., Dykes, Gary A. and Rahman, Sadequr 2016, Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia, Journal of infection and public health, vol. 10, no. 5, pp. 617-623, doi: 10.1016/j.jiph.2017.02.006.

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Title Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia
Author(s) Daniel, Diane S.
Lee, Sui M.
Gan, Han M.
Dykes, Gary A.
Rahman, Sadequr
Journal name Journal of infection and public health
Volume number 10
Issue number 5
Start page 617
End page 623
Total pages 7
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-10-14
ISSN 1876-0341
1876-035X
Keyword(s) antibiotic resistance
Enterococcus faecalis
genetic diversity
molecular typing
virulence markers
Summary Enterococcus faecalis ranks as one of the leading causes of nosocomial infections. A strong epidemiological link has been reported between E. faecalis inhabiting animals and environmental sources. This study investigates the genetic diversity, antibiotic resistance and virulence determinants in E. faecalis from three sources in Malaysia. A total of 250 E. faecalis isolates were obtained consisting of 120 isolates from farm animals, 100 isolates from water sources and 30 isolates from hospitalized patients. Pulse-field gel electrophoresis-typing yielded 63 pulsotypes, with high diversity observed in all sources (D = ≥0.901). No pulsotype was common to all the three sources. Each patient room had its own unique PFGE pattern which persisted after six months. Minimum inhibitory concentrations of Vancomycin, Gentamicin, Penicillin, Tetracycline, Nitrofurantoin, Levofloxacin, Ciprofloxacin and Fosfomycin were evaluated. Resistance to Tetracycline was most prevalent in isolates from farm animals (62%) and water sources (49%). Water isolates (86%) had a higher prevalence of the asa1 gene, which encodes for aggregation substance, whereas clinical (78%) and farm animal isolates (87%) had a higher prevalence of the esp gene, encoding a surface exposed protein. This study generates knowledge on the genetic diversity of E. faecalis with antibiotic resistance and virulence characteristics from various sources in Malaysia.
Language eng
DOI 10.1016/j.jiph.2017.02.006
Field of Research 060408 Genomics
1117 Public Health And Health Services
1199 Other Medical And Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution Non-Commercial No-Derivatives licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30101951

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.