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Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels

Nolan, Laura M., Beatson, Scott A., Croft, Larry, Jones, Peter M., George, Anthony M., Mattick, John S., Turnbull, Lynne and Whitchurch, Cynthia B. 2012, Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels, Microbiologyopen, vol. 1, no. 4, pp. 490-501, doi: 10.1002/mbo3.49.

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Title Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels
Formatted title Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels
Author(s) Nolan, Laura M.
Beatson, Scott A.
Croft, Larry
Jones, Peter M.
George, Anthony M.
Mattick, John S.
Turnbull, Lynne
Whitchurch, Cynthia B.
Journal name Microbiologyopen
Volume number 1
Issue number 4
Start page 490
End page 501
Total pages 12
Publisher Wiley
Place of publication Chichester, Eng.
Publication date 2012-12
ISSN 2045-8827
Keyword(s) Amino Acid Sequence
Cyclic AMP
DNA, Bacterial
Fimbriae Proteins
Fimbriae, Bacterial
Genome, Bacterial
Phenotype
Polymerase Chain Reaction
Pseudomonas aeruginosa
Sequence Alignment
Sequence Analysis, DNA
Suppression, Genetic
Summary Cyclic AMP (cAMP) is a signaling molecule that is involved in the regulation of multiple virulence systems of the opportunistic pathogen Pseudomonas aeruginosa. The intracellular concentration of cAMP in P. aeruginosa cells is tightly controlled at the levels of cAMP synthesis and degradation through regulation of the activity and/or expression of the adenylate cyclases CyaA and CyaB or the cAMP phosphodiesterase CpdA. Interestingly, mutants of fimL, which usually demonstrate defective twitching motility, frequently revert to a wild‐type twitching‐motility phenotype presumably via the acquisition of an extragenic suppressor mutation(s). In this study, we have characterized five independent fimL twitching‐motility revertants and have determined that all have increased intracellular cAMP levels compared with the parent fimL mutant. Whole‐genome sequencing revealed that only one of these fimL revertants has acquired a loss‐of‐function mutation in cpdA that accounts for the elevated levels of intracellular cAMP. As mutation of cpdA did not account for the restoration of twitching motility observed in the other four fimL revertants, these observations suggest that there is at least another, as yet unidentified, site of extragenic suppressor mutation that can cause phenotypic reversion in fimL mutants and modulation of intracellular cAMP levels of P. aeruginosa.
Language eng
DOI 10.1002/mbo3.49
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30106042

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.