Openly accessible

MicroRNAs in the shoot apical meristem of soybean

Wong, Chui E., Zhao, Ying-Tao, Wang, Xiu-Jie, Croft, Larry, Wang, Zhong-Hua, Haerizadeh, Farzad, Mattick, John S., Singh, Mohan B., Carroll, Bernard J. and Bhalla, Prem L. 2011, MicroRNAs in the shoot apical meristem of soybean, Journal of experimental botany, vol. 62, no. 8, pp. 2495-2506, doi: 10.1093/jxb/erq437.

Attached Files
Name Description MIMEType Size Downloads
croft-micrornasin-2011.pdf Published version application/pdf 876.03KB 4

Title MicroRNAs in the shoot apical meristem of soybean
Author(s) Wong, Chui E.
Zhao, Ying-Tao
Wang, Xiu-Jie
Croft, Larry
Wang, Zhong-Hua
Haerizadeh, Farzad
Mattick, John S.
Singh, Mohan B.
Carroll, Bernard J.
Bhalla, Prem L.
Journal name Journal of experimental botany
Volume number 62
Issue number 8
Start page 2495
End page 2506
Total pages 12
Publisher Oxford Academic
Place of publication Oxford, Eng.
Publication date 2011-05
ISSN 1460-2431
Keyword(s) Base Sequence
Conserved Sequence
Gene Expression Regulation, Plant
In Situ Hybridization
Meristem
MicroRNAs
Molecular Sequence Data
Plant Leaves
RNA, Messenger
RNA, Plant
Sequence Analysis, DNA
Soybeans
Summary Plant microRNAs (miRNAs) play crucial regulatory roles in various developmental processes. In this study, we characterize the miRNA profile of the shoot apical meristem (SAM) of an important legume crop, soybean, by integrating high-throughput sequencing data with miRNA microarray analysis. A total of 8423 non-redundant sRNAs were obtained from two libraries derived from micro-dissected SAM or mature leaf tissue. Sequence analysis allowed the identification of 32 conserved miRNA families as well as 8 putative novel miRNAs. Subsequent miRNA profiling with microarrays verified the expression of the majority of these conserved and novel miRNAs. It is noteworthy that several miRNAs* were expressed at a level similar to or higher than their corresponding mature miRNAs in SAM or mature leaf, suggesting a possible biological function for the star species. In situ hybridization analysis revealed a distinct spatial localization pattern for a conserved miRNA, miR166, and its star speciessuggesting that they serve different roles in regulating leaf development. Furthermore, localization studies showed that a novel soybean miRNA, miR4422a, was nuclear-localized. This study also indicated a novel expression pattern of miR390 in soybean. Our approach identified potential key regulators and provided vital spatial information towards understanding the regulatory circuits in the SAM of soybean during shoot development.
Language eng
DOI 10.1093/jxb/erq437
Field of Research 0607 Plant Biology
0703 Crop And Pasture Production
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution non-commercial licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30106047

Document type: Journal Article
Collections: School of Life and Environmental Sciences
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 37 times in TR Web of Science
Scopus Citation Count Cited 44 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 10 Abstract Views, 5 File Downloads  -  Detailed Statistics
Created: Tue, 17 Apr 2018, 17:07:43 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.