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High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia

Drewes, Julia L., White, James R., Dejea, Christine M., Fathi, Payam, Iyadorai, Thevambiga, Vadivelu, Jamuna, Roslani, April C., Wick, Elizabeth C., Mongodin, Emmanuel F., Loke, Mun Fai, Thulasi, Kumar, Gan, Han Ming, Goh, Khean Lee, Chong, Hoong Yin, Kumar, Sandip, Wanyiri, Jane W. and Sears, Cynthia L. 2017, High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia, npj biofilms and microbiomes, vol. 3, doi: 10.1038/s41522-017-0040-3.

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Title High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia
Author(s) Drewes, Julia L.
White, James R.
Dejea, Christine M.
Fathi, Payam
Iyadorai, Thevambiga
Vadivelu, Jamuna
Roslani, April C.
Wick, Elizabeth C.
Mongodin, Emmanuel F.
Loke, Mun Fai
Thulasi, Kumar
Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Goh, Khean Lee
Chong, Hoong Yin
Kumar, Sandip
Wanyiri, Jane W.
Sears, Cynthia L.
Journal name npj biofilms and microbiomes
Volume number 3
Article ID 34
Total pages 12
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2017-12-01
ISSN 2055-5008
2055-5008
Summary © 2017 The Author(s). Colorectal cancer (CRC) remains the third most common cancer worldwide, with a growing incidence among young adults. Multiple studies have presented associations between the gut microbiome and CRC, suggesting a link with cancer risk. Although CRC microbiome studies continue to profile larger patient cohorts with increasingly economical and rapid DNA sequencing platforms, few common associations with CRC have been identified, in part due to limitations in taxonomic resolution and differences in analysis methodologies. Complementing these taxonomic studies is the newly recognized phenomenon that bacterial organization into biofilm structures in the mucus layer of the gut is a consistent feature of right-sided (proximal), but not left-sided (distal) colorectal cancer. In the present study, we performed 16S rRNA gene amplicon sequencing and biofilm quantification in a new cohort of patients from Malaysia, followed by a meta-analysis of eleven additional publicly available data sets on stool and tissue-based CRC microbiota using Resphera Insight, a high-resolution analytical tool for species-level characterization. Results from the Malaysian cohort and the expanded meta-analysis confirm that CRC tissues are enriched for invasive biofilms (particularly on right-sided tumors), a symbiont with capacity for tumorigenesis (Bacteroides fragilis), and oral pathogens including Fusobacterium nucleatum, Parvimonas micra, and Peptostreptococcus stomatis. Considered in aggregate, species from the Human Oral Microbiome Database are highly enriched in CRC. Although no detected microbial feature was universally present, their substantial overlap and combined prevalence supports a role for the gut microbiota in a significant percentage ( > 80%) of CRC cases.
Language eng
DOI 10.1038/s41522-017-0040-3
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30107527

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.