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Anti-coagulant and anti-thrombotic properties of blacklip abalone (Haliotis rubra): in vitro and animal studies

Suleria, Hafiz Ansar Rasul, Masci, Paul P., Zhao, Kong-Nan, Addepalli, Rama, Chen, Wei, Osborne, Simone A. and Gobe, Glenda C. 2017, Anti-coagulant and anti-thrombotic properties of blacklip abalone (Haliotis rubra): in vitro and animal studies, Marine drugs, vol. 15, no. 8, Special Issue: Target Identification of Marine Products, pp. 1-10, doi: 10.3390/md15080240.

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Title Anti-coagulant and anti-thrombotic properties of blacklip abalone (Haliotis rubra): in vitro and animal studies
Formatted title Anti-coagulant and anti-thrombotic properties of blacklip abalone (Haliotis rubra): in vitro and animal studies
Author(s) Suleria, Hafiz Ansar RasulORCID iD for Suleria, Hafiz Ansar Rasul orcid.org/0000-0002-2450-0830
Masci, Paul P.
Zhao, Kong-Nan
Addepalli, Rama
Chen, Wei
Osborne, Simone A.
Gobe, Glenda C.
Journal name Marine drugs
Volume number 15
Issue number 8
Season Special Issue: Target Identification of Marine Products
Article ID 240
Start page 1
End page 10
Total pages 10
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2017-08
ISSN 1660-3397
1660-3397
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Chemistry, Medicinal
Pharmacology & Pharmacy
marine processing waste
blacklip abalone
anti-thrombotic and anti-coagulant activity
SULFATED FUCANS
ANTICOAGULANT
GLYCOSAMINOGLYCAN
PHARMACOKINETICS
POLYSACCHARIDE
NANOPARTICLES
PERSPECTIVES
THROMBOSIS
WARFARIN
HEPARIN
Summary Sulphated polysaccharides with anti-thrombotic and anti-coagulant activities have been found in various marine biota. In this study, a previously characterised anti-thrombotic and anti-coagul ant extract from blacklip abalone was fractionated by anion exchange chromatography (AEC), pooled (on a sulphated polysaccharide basis) and administered to Wistar rats via oral gavage (N = 8) for assessment as an oral therapeutic. To ensure that the preparation had anti-coagulant activity prior to oral administration, it was assessed in rat blood by thromboelastography (TEG) significantly increasing reaction (R) time (or time until clot formation). Following in vitro confirmation of anti-coagulant activity, 40 mg of the preparation was orally administered to rats with blood samples collected at 2, 4, and 6 h post-gavage. Assessment of all blood samples by TEG showed some prolongation of R time from 355 to 380 s after 4 h. Dosing of the post-gavage blood samples with the abalone preparation to confirm anti-thrombotic activity in vitro revealed residual anti-coagulant activity, further suggesting that oral administration did increase anti-coagulant potential in the collected blood but that bioavailability was low. Assessment of tissues and haematological parameters showed no obvious harmful effects of the abalone preparation in animals. In summary, even though oral administration of fractionated and pooled blacklip abalone extract to rats delayed clotting after 4 h, bioavailability of the preparation appeared to be low and may be more appropriate for intravenous administration as an anti-thrombotic or anti-coagulant therapeutic.
Language eng
DOI 10.3390/md15080240
Field of Research 1115 Pharmacology And Pharmaceutical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, by the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30107918

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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