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In vitro anti-thrombotic activity of extracts from blacklip abalone (Haliotis rubra) processing waste

Suleria, Hafiz Ansar Rasul, Hines, Barney M, Addepalli, Rama, Chen, Wei, Masci, Paul, Gobe, Glenda and Osborne, Simone A 2017, In vitro anti-thrombotic activity of extracts from blacklip abalone (Haliotis rubra) processing waste, Marine drugs, vol. 15, no. 1, pp. 1-17, doi: 10.3390/md15010008.

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Title In vitro anti-thrombotic activity of extracts from blacklip abalone (Haliotis rubra) processing waste
Formatted title In vitro anti-thrombotic activity of extracts from blacklip abalone (Haliotis rubra) processing waste
Author(s) Suleria, Hafiz Ansar RasulORCID iD for Suleria, Hafiz Ansar Rasul orcid.org/0000-0002-2450-0830
Hines, Barney M
Addepalli, Rama
Chen, Wei
Masci, Paul
Gobe, Glenda
Osborne, Simone A
Journal name Marine drugs
Volume number 15
Issue number 1
Article ID 8
Start page 1
End page 17
Total pages 17
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2017-01
ISSN 1660-3397
1660-3397
Keyword(s) anti-thrombotic activity
bioactive molecules
blacklip abalone
processing waste
animals
anticoagulants
aquatic organisms
blood coagulation tests
fibrinolytic agents
gastropoda
heparin cofactor II
partial thromboplastin time
polysaccharides
prothrombin time
thrombin
thrombosis
Summary Waste generated from the processing of marine organisms for food represents an underutilized resource that has the potential to provide bioactive molecules with pharmaceutical applications. Some of these molecules have known anti-thrombotic and anti-coagulant activities and are being investigated as alternatives to common anti-thrombotic drugs, like heparin and warfarin that have serious side effects. In the current study, extracts prepared from blacklip abalone (Haliotis rubra) processing waste, using food grade enzymes papain and bromelain, were found to contain sulphated polysaccharide with anti-thrombotic activity. Extracts were found to be enriched with sulphated polysaccharides and assessed for anti-thrombotic activity in vitro through heparin cofactor-II (HCII)-mediated inhibition of thrombin. More than 60% thrombin inhibition was observed in response to 100 μg/mL sulphated polysaccharides. Anti-thrombotic potential was further assessed as anti-coagulant activity in plasma and blood, using prothrombin time (PT), activated partial thromboplastin time (aPTT), and thromboelastography (TEG). All abalone extracts had significant activity compared with saline control. Anion exchange chromatography was used to separate extracts into fractions with enhanced anti-thrombotic activity, improving HCII-mediated thrombin inhibition, PT and aPTT almost 2-fold. Overall this study identifies an alternative source of anti-thrombotic molecules that can be easily processed offering alternatives to current anti-thrombotic agents like heparin.
Language eng
DOI 10.3390/md15010008
Field of Research 1115 Pharmacology And Pharmaceutical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30107943

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.