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A new method for targeted and sustained induction of type 2 diabetes in rodents

Premilovac, Dino, Gasperini, Robert J, Sawyer, Sarah, West, Adrian, Keske, Michelle A, Taylor, Bruce V and Foa, Lisa 2017, A new method for targeted and sustained induction of type 2 diabetes in rodents, Scientific reports, vol. 7, pp. 1-10, doi: 10.1038/s41598-017-14114-4.

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Title A new method for targeted and sustained induction of type 2 diabetes in rodents
Author(s) Premilovac, Dino
Gasperini, Robert J
Sawyer, Sarah
West, Adrian
Keske, Michelle AORCID iD for Keske, Michelle A orcid.org/0000-0003-4214-7628
Taylor, Bruce V
Foa, Lisa
Journal name Scientific reports
Volume number 7
Article ID 14158
Start page 1
End page 10
Total pages 10
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2017-10-26
ISSN 2045-2322
Keyword(s) type 2 diabetes
metabolic disorder
morbidity
mortality
rodents
science & technology
multidisciplinary sciences
Summary Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80-200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans.
Language eng
DOI 10.1038/s41598-017-14114-4
Field of Research 110306 Endocrinology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30108714

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.