Openly accessible

Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita

Rocha, Thales L., Soll, Carla Becker, Boughton, Berin A., Silva, Tiago S., Oldach, Klaus, Firmino, Alexandre A. P., Callahan, Damien L., Sheedy, John, Silveira, Edilberto R., Carneiro, Regina M. D. G., Silva, Luciano P., Polez, Vera L. P., Pelegrini, Patricia B., Bacic, Antony, Grossi-de-Sa, Maria F. and Roessner, Ute 2017, Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita, Biotechnology research and innovation, vol. 1, no. 1, pp. 87-100, doi: 10.1016/j.biori.2017.10.003.

Attached Files
Name Description MIMEType Size Downloads
callahan-prospectionandidentif-2017.pdf Published version application/pdf 1.12MB 1

Title Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita
Formatted title Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita
Author(s) Rocha, Thales L.
Soll, Carla Becker
Boughton, Berin A.
Silva, Tiago S.
Oldach, Klaus
Firmino, Alexandre A. P.
Callahan, Damien L.ORCID iD for Callahan, Damien L. orcid.org/0000-0002-6384-8717
Sheedy, John
Silveira, Edilberto R.
Carneiro, Regina M. D. G.
Silva, Luciano P.
Polez, Vera L. P.
Pelegrini, Patricia B.
Bacic, Antony
Grossi-de-Sa, Maria F.
Roessner, Ute
Journal name Biotechnology research and innovation
Volume number 1
Issue number 1
Start page 87
End page 100
Total pages 14
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2017-01
ISSN 2452-0721
Summary Meloidogyne incognita is responsible for significant crop losses worldwide. The main strategy to control this phytopathogen is still based on synthetic nematicides that are harmful to human health and the environment. In this context, aqueous seed extracts of antagonistic plants were screened for molecules effective toward the infective stage (J2) of M. incognita. The aqueous crude extract of Canavalia ensiformis (ACECe) showed the highest nematicidal activity (87 ± 3% mortality). ACECe dialysis fractionation allowed the collection of an external dialysate (EDCe) containing molecules smaller than 3.5 kDa effective against J2 (96 ± 3.0% mortality); innocuous toward non targeted organisms as saprophytic nematodes, fungi, bacterium and insects larvae; active against J2 (96 ± 2% mortality) after heating (50 °C); no cytotoxic for bovine red blood cells; reduction of M. incognita eggs masses by 82.5% in tomato plants at green house conditions. Fractionation of the EDCe by reversed-phase high-performance liquid chromatography (RP-HPLC) separated five nematotoxic fractions. Analyses of those fractions based on gas chromatography–mass spectrometry (GC–MS), liquid chromatography–mass spectrometry (LCMS) and nuclear magnetic resonance (NMR) identified nine specific metabolites, follow-up testing of the individual authentic standards of each metabolite as the compounds: d-glucose, l-canavanine, xanthotoxin, cis-aconitic acid, trans-aconitic acid, malic acid, citric acid, palmitic acid and S-carboxymethylcysteine; all them confirmed to possess nematotoxic properties. However, some of those metabolites were not described previously demonstrating biological action against M. incognita.
Language eng
DOI 10.1016/j.biori.2017.10.003
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, Sociedade Brasileira de Biotecnologia
Free to Read? Yes
Use Rights Creative Commons Attribution Non-Commercial No-Derivatives licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30108954

Document type: Journal Article
Collections: School of Life and Environmental Sciences
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 9 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Fri, 01 Jun 2018, 16:47:00 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.