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Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells

Islam, Rabia, Yoon, Heein, Kim, Bong-Soo, Bae, Han-Sol, Shin, Hye-Rim, Kim, Woo-Jin, Yoon, Won-Joon, Lee, Yun-Sil, Woo, Kyung Mi, Baek, Jeong-Hwa and Ryoo, Hyun-Mo 2017, Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells, Scientific reports, vol. 7, pp. 1-12, doi: 10.1038/s41598-017-07229-1.

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Title Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
Author(s) Islam, RabiaORCID iD for Islam, Rabia orcid.org/0000-0001-7378-9071
Yoon, Heein
Kim, Bong-Soo
Bae, Han-Sol
Shin, Hye-Rim
Kim, Woo-Jin
Yoon, Won-Joon
Lee, Yun-Sil
Woo, Kyung Mi
Baek, Jeong-Hwa
Ryoo, Hyun-Mo
Journal name Scientific reports
Volume number 7
Article ID 6977
Start page 1
End page 12
Total pages 12
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2017-08-01
ISSN 2045-2322
Keyword(s) blood-testis barrier
Pin1
Sertoli cells
serine
threonine-phosphorylated proteins
prolyl isomerase
isomerization
Ser/Thr-Pro bonds
post mitotic cell
activin pathways
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SPERMATOGONIAL STEM-CELLS
ANCHORING JUNCTIONS
MOUSE TESTIS
LEYDIG-CELLS
MICE
SPERMATOGENESIS
WT1
DIFFERENTIATION
PROLIFERATION
INFERTILITY
Summary The conformation and function of a subset of serine and threonine-phosphorylated proteins are regulated by the prolyl isomerase Pin1 through isomerization of phosphorylated Ser/Thr-Pro bonds. Pin1 is intensely expressed in Sertoli cells, but its function in this post mitotic cell remains unclear. Our aim was to investigate the role of Pin1 in the Sertoli cells. Lack of Pin1 caused disruption of the blood-testis barrier. We next investigated if the activin pathways in the Sertoli cells were affected by lack of Pin1 through immunostaining for Smad3 protein in testis tissue. Indeed, lack of Pin1 caused reduced Smad3 expression in the testis tissue, as well as a reduction in the level of N-Cadherin, a known target of Smad3. Pin1−/− testes express Sertoli cell marker mRNAs in a pattern similar to that seen in Smad3+/− mice, except for an increase in Wt1 expression. The resulting dysregulation of N-Cadherin, connexin 43, and Wt1 targets caused by lack of Pin1 might affect the mesenchymal–epithelial balance in the Sertoli cells and perturb the blood-testis barrier. The effect of Pin1 dosage in Sertoli cells might be useful in the study of toxicant-mediated infertility, gonadal cancer, and for designing male contraceptives.
Language eng
DOI 10.1038/s41598-017-07229-1
Field of Research 110399 Clinical Sciences not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30109121

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.