Development of cell-specific aptamers: recent advances and insight into the selection procedures

Rahimizadeh, Kamal, AlShamaileh, Hadi, Fratini, Milena, Chakravarthy, Madhuri, Stephen, Michelle, Shigdar, Sarah and Veedu, Rakesh N. 2017, Development of cell-specific aptamers: recent advances and insight into the selection procedures, Molecules, vol. 22, no. 12, doi: 10.3390/molecules22122070.

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Title Development of cell-specific aptamers: recent advances and insight into the selection procedures
Author(s) Rahimizadeh, Kamal
AlShamaileh, Hadi
Fratini, Milena
Chakravarthy, Madhuri
Stephen, Michelle
Shigdar, SarahORCID iD for Shigdar, Sarah orcid.org/0000-0001-8084-8447
Veedu, Rakesh N.
Journal name Molecules
Volume number 22
Issue number 12
Total pages 9
Publisher M D P I
Place of publication Basel, Switzerland
Publication date 2017-11-27
ISSN 1420-3049
Keyword(s) aptamers
Cell-SELEX
aptamer enrichment
ssDNA
binding assays
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
Chemistry
SINGLE-STRANDED-DNA
COLORECTAL-CANCER CELLS
SELEX-BASED SELECTION
RNA APTAMERS
EXPONENTIAL ENRICHMENT
SYSTEMATIC EVOLUTION
ASYMMETRIC PCR
IN-VITRO
MOLECULAR RECOGNITION
LIGANDS
Summary Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.
Language eng
DOI 10.3390/molecules22122070
Field of Research 0305 Organic Chemistry
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30109407

Document type: Journal Article
Collection: School of Medicine
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