Development of cell-specific aptamers: recent advances and insight into the selection procedures

Rahimizadeh, Kamal, AlShamaileh, Hadi, Fratini, Milena, Chakravarthy, Madhuri, Stephen, Michelle, Shigdar, Sarah and Veedu, Rakesh N. 2017, Development of cell-specific aptamers: recent advances and insight into the selection procedures, Molecules, vol. 22, no. 12, doi: 10.3390/molecules22122070.

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Title Development of cell-specific aptamers: recent advances and insight into the selection procedures
Author(s) Rahimizadeh, Kamal
AlShamaileh, Hadi
Fratini, Milena
Chakravarthy, Madhuri
Stephen, Michelle
Shigdar, SarahORCID iD for Shigdar, Sarah
Veedu, Rakesh N.
Journal name Molecules
Volume number 22
Issue number 12
Total pages 9
Publisher M D P I
Place of publication Basel, Switzerland
Publication date 2017-11-27
ISSN 1420-3049
Keyword(s) aptamers
aptamer enrichment
binding assays
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
Summary Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.
Language eng
DOI 10.3390/molecules22122070
Field of Research 0305 Organic Chemistry
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
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Document type: Journal Article
Collection: School of Medicine
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