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Electrophysiological evidence of RML12 mosquito cell line towards neuronal differentiation by 20-hydroxyecdysdone

Gaburro, Julie, Duchemin, Jean-Bernard, Paradkar, Prasad N, Nahavandi, Saeid N and Bhatti, Asim 2018, Electrophysiological evidence of RML12 mosquito cell line towards neuronal differentiation by 20-hydroxyecdysdone, Scientific reports, vol. 8, pp. 1-11, doi: 10.1038/s41598-018-28357-2.

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Title Electrophysiological evidence of RML12 mosquito cell line towards neuronal differentiation by 20-hydroxyecdysdone
Author(s) Gaburro, JulieORCID iD for Gaburro, Julie orcid.org/0000-0001-6609-6429
Duchemin, Jean-Bernard
Paradkar, Prasad N
Nahavandi, Saeid NORCID iD for Nahavandi, Saeid N orcid.org/0000-0002-0360-5270
Bhatti, AsimORCID iD for Bhatti, Asim orcid.org/0000-0001-6876-1437
Journal name Scientific reports
Volume number 8
Article ID 10109
Start page 1
End page 11
Total pages 11
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2018-07-04
ISSN 2045-2322
Keyword(s) differentiation
extracellular recording
science & technology
Summary Continuous cell lines from insect larval tissues are widely used in different research domains, such as virology, insect immunity, gene expression, and bio pharmacology. Previous study showed that introduction of 20-hydroxyecdysone to Spodoptera cell line induced a neuron-like morphology with neurite extensions. Despite some results suggesting potential presence of neuro-receptors, no study so far has shown that these neuron-induced cells were functional. Here, using microelectrode arrays, we showed that the mosquito cell line, RML12, differentiated with 20-hydroxyecdysone, displays spontaneous electrophysiological activity. Results showed that these cells can be stimulated by GABAergic antagonist as well as nicotinic agonist. These results provide new evidence of neuron-like functionality of 20-hydroxyecdysone induced differentiated mosquito cell line. Finally, we used this new model to test the effects of two insecticides, temephos and permethrin. Our analysis revealed significant changes in the spiking activity after the introduction of these insecticides with prolonged effect on the neuronal activity. We believe that this differentiated mosquito neuronal cell model can be used for high-throughput screening of new pesticides on insect nervous system instead of primary neurons or in vivo studies.
Language eng
DOI 10.1038/s41598-018-28357-2
Field of Research 090303 Biomedical Instrumentation
090609 Signal Processing
060102 Bioinformatics
060101 Analytical Biochemistry
100402 Medical Biotechnology Diagnostics (incl Biosensors)
100703 Nanobiotechnology
110902 Cellular Nervous System
Socio Economic Objective 970109 Expanding Knowledge in Engineering
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2018, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30109967

Document type: Journal Article
Collections: Centre for Intelligent Systems Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.