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Comparison of solvate ionic liquids and DMSO as an in vivo delivery and storage media for small molecular therapeutics

Yoganantharajah, Prusothman, Ray, Alexander P, Eyckens, Daniel J, Henderson, Luke C and Gibert, Yann 2018, Comparison of solvate ionic liquids and DMSO as an in vivo delivery and storage media for small molecular therapeutics, BMC biotechnology, vol. 18, pp. 1-7, doi: 10.1186/s12896-018-0442-1.

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Title Comparison of solvate ionic liquids and DMSO as an in vivo delivery and storage media for small molecular therapeutics
Author(s) Yoganantharajah, Prusothman
Ray, Alexander P
Eyckens, Daniel J
Henderson, Luke CORCID iD for Henderson, Luke C orcid.org/0000-0002-4244-2056
Gibert, Yann
Journal name BMC biotechnology
Volume number 18
Article ID 32
Start page 1
End page 7
Total pages 7
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2018-05-29
ISSN 1472-6750
1472-6750
Keyword(s) zebrafish
embryogenesis
retinoic acid
Aldh1a2
FGFR
DEAB
SU5402
ionic liquids
science & technology
life sciences & biomedicine
biotechnology & applied microbiology
Summary BACKGROUND: Solvate ionic liquids (SILs) are a new class of ionic liquids that are equimolar solutions of lithium bistrifluoromethanesulfonimide in either triglyme or tetraglyme, referred to as G3LiTFSA and G4LiTFSA, respectively. SILs play a role in energy storage lithium batteries, and have been proposed as potential alternatives to traditional organic solvents such as DMSO. G3TFSA and G4TFSA have been shown to exhibit no toxicity in vivo up to 0.5% (v/v), and solubilize small compounds (N,N-diethylaminobenzaldehyde) with full penetrance, similar to DMSO delivered DEAB. Herein, we compare the effects of storage (either at room temperature or - 20 °C) on DEAB solubilized in either DMSO, G3TFSA or G4TFSA to investigate compound degradation and efficacy.

RESULTS: The findings show that DEAB stored at room temperature (RT) for 4 months solubilized in either G3TFSA, G4TFSA or DMSO displayed no loss of penetrance. The same was observed with stock solutions stored at - 20 °C for 4 months; however G4TFSA remained in a liquid state compared to both G3TFSA and DMSO. Moreover, we examined the ability of G3TFSA and G4TFSA to solubilize another small molecular therapeutic, the FGFR antagonist SU5402. G4TFSA, unlike G3TFSA solubilized SU5402 and displayed similar phenotypic characteristics and reduced dlx2a expression as reported and shown with SU5402 in DMSO; albeit more penetrative.

CONCLUSION: This study validates the use of these ionic liquids as a potential replacement for DMSO in vivo as organic solubilizing agents.
Language eng
DOI 10.1186/s12896-018-0442-1
Field of Research 030306 Synthesis of Materials
06 Biological Sciences
10 Technology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2018, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30110240

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.