Openly accessible

Longitudinal, genome-scale analysis of DNA methylation in twins from birth to 18 months of age reveals rapid epigenetic change in early life and pair-specific effects of discordance

Martino, David, Loke, Yuk Jin, Gordon, Lavinia, Ollikainen, Miina, Cruickshank, Mark N., Saffery, Richard and Craig, Jeffrey M. 2013, Longitudinal, genome-scale analysis of DNA methylation in twins from birth to 18 months of age reveals rapid epigenetic change in early life and pair-specific effects of discordance, Genome Biology, vol. 14, no. 5, pp. R42, doi: 10.1186/gb-2013-14-5-r42.

Attached Files
Name Description MIMEType Size Downloads
craig-longitudinalgenomescale-2013.pdf Published version application/pdf 2.39MB 2

Title Longitudinal, genome-scale analysis of DNA methylation in twins from birth to 18 months of age reveals rapid epigenetic change in early life and pair-specific effects of discordance
Author(s) Martino, David
Loke, Yuk Jin
Gordon, Lavinia
Ollikainen, Miina
Cruickshank, Mark N.
Saffery, Richard
Craig, Jeffrey M.
Journal name Genome Biology
Volume number 14
Issue number 5
Start page R42
Total pages 14
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2013-05-22
ISSN 1474-760X
1474-760X
Keyword(s) Age Factors
CpG Islands
DNA
DNA Methylation
Epigenesis, Genetic
Epigenomics
Female
Genome, Human
Humans
Infant
Infant, Newborn
Longitudinal Studies
Male
Mouth
Pilot Projects
Twins
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
CPG ISLAND SHORES
MONOZYGOTIC TWINS
GESTATIONAL-AGE
MICROARRAY DATA
SHARED ENVIRONMENT
COMPLEX TRAITS
HUMAN BRAIN
STEM-CELLS
TISSUE
PROFILE
Summary BACKGROUND: The extent to which development- and age-associated epigenetic changes are influenced by genetic, environmental and stochastic factors remains to be discovered. Twins provide an ideal model with which to investigate these influences but previous cross-sectional twin studies provide contradictory evidence of within-pair epigenetic drift over time. Longitudinal twin studies can potentially address this discrepancy.

RESULTS: In a pilot, genome-scale study of DNA from buccal epithelium, a relatively homogeneous tissue, we show that one-third of the CpGs assayed show dynamic methylation between birth and 18 months. Although all classes of annotated genomic regions assessed show an increase in DNA methylation over time, probes located in intragenic regions, enhancers and low-density CpG promoters are significantly over-represented, while CpG islands and high-CpG density promoters are depleted among the most dynamic probes. Comparison of co-twins demonstrated that within-pair drift in DNA methylation in our cohort is specific to a subset of pairs, who show more differences at 18 months. The rest of the pairs show either minimal change in methylation discordance, or more similar, converging methylation profiles at 18 months. As with age-associated regions, sites that change in their level of within-pair discordance between birth and 18 months are enriched in genes involved in development, but the average magnitude of change is smaller than for longitudinal change.

CONCLUSIONS: Our findings suggest that DNA methylation in buccal epithelium is influenced by non-shared stochastic and environmental factors that could reflect a degree of epigenetic plasticity within an otherwise constrained developmental program.
Language eng
DOI 10.1186/gb-2013-14-5-r42
Field of Research 05 Environmental Sciences
06 Biological Sciences
08 Information And Computing Sciences
Copyright notice ©2013, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30110745

Document type: Journal Article
Collections: School of Medicine
Open Access Collection
Elements - New records (hidden)
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 101 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 7 Abstract Views, 3 File Downloads  -  Detailed Statistics
Created: Tue, 10 Jul 2018, 10:57:13 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.