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Rationally designed probe for reversible sensing of zinc and application in cells

Heng, Sabrina, Reineck, Philipp, Vidanapathirana, Achini K., Pullen, Benjamin J., Drumm, Daniel W., Ritter, Lesley J., Schwarz, Nisha, Bonder, Claudine S., Psaltis, Peter J., Thompson, Jeremy G., Gibson, Brant C., Nicholls, Stephen J. and Abell, Andrew D. 2017, Rationally designed probe for reversible sensing of zinc and application in cells, ACS omega, vol. 2, no. 9, pp. 6201-6210, doi: 10.1021/acsomega.7b00923.

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Title Rationally designed probe for reversible sensing of zinc and application in cells
Author(s) Heng, Sabrina
Reineck, Philipp
Vidanapathirana, Achini K.
Pullen, Benjamin J.
Drumm, Daniel W.ORCID iD for Drumm, Daniel W. orcid.org/0000-0001-5663-1387
Ritter, Lesley J.
Schwarz, Nisha
Bonder, Claudine S.
Psaltis, Peter J.
Thompson, Jeremy G.
Gibson, Brant C.
Nicholls, Stephen J.
Abell, Andrew D.
Journal name ACS omega
Volume number 2
Issue number 9
Start page 6201
End page 6210
Total pages 10
Publisher ACS Publications
Place of publication Washington, D.C.
Publication date 2017-09
ISSN 2470-1343
Summary Biologically compatible fluorescent ion sensors, particularly those that are reversible, represent a key tool for answering a range of fundamental biological questions. We report a rationally designed probe with a 6′-fluoro spiropyran scaffold (5) for the reversible sensing of zinc (Zn2+) in cells. The 6′-fluoro substituent overcomes several limitations normally associated with spiropyran-based sensors to provide an improved signal-To-background ratio and faster photoswitching times in aqueous solution. In vitro studies were performed with 5 and the 6′-nitro analogues (6) in HEK 293 and endothelial cells. The new spiropyran (5) can detect exogenous Zn2+inside both cell types and without affecting the proliferation of endothelial cells. Studies were also performed on dying HEK 293 cells, with results demonstrating the ability of the key compound to detect endogenous Zn2+efflux from cells undergoing apoptosis. Biocompatibility and photoswitching of 5 were demonstrated within endothelial cells but not with 6, suggesting the future applicability of sensor 5 to study intracellular Zn2+efflux in these systems.
Language eng
DOI 10.1021/acsomega.7b00923
Field of Research 030199 Analytical Chemistry not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, American Chemical Society
Free to Read? Yes
Use Rights https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
Persistent URL http://hdl.handle.net/10536/DRO/DU:30110801

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.