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Immunological comparison of native and recombinant hen's egg yolk allergen, chicken serum albumin (Gal d 5), produced in Kluveromyces lactis

De Silva, Chamika, Dhanapala, Pathum, King, Samuel, Doran, Timothy, Tang, Mimi and Suphioglu, Cenk 2018, Immunological comparison of native and recombinant hen's egg yolk allergen, chicken serum albumin (Gal d 5), produced in Kluveromyces lactis, Nutrients, vol. 10, no. 6, pp. 1-11, doi: 10.3390/nu10060757.

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Title Immunological comparison of native and recombinant hen's egg yolk allergen, chicken serum albumin (Gal d 5), produced in Kluveromyces lactis
Formatted title Immunological comparison of native and recombinant hen's egg yolk allergen, chicken serum albumin (Gal d 5), produced in Kluveromyces lactis
Author(s) De Silva, Chamika
Dhanapala, Pathum
King, Samuel
Doran, Timothy
Tang, Mimi
Suphioglu, CenkORCID iD for Suphioglu, Cenk orcid.org/0000-0003-0101-0668
Journal name Nutrients
Volume number 10
Issue number 6
Article ID 757
Start page 1
End page 11
Total pages 11
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2018-06-12
ISSN 2072-6643
Keyword(s) allergy
egg yolk allergens
recombinant allergens
chicken serum albumin
allergens
animals
antibody specificity
biomarkers
chickens
egg hypersensitivity
egg proteins, dietary
epitopes
humans
immunoglobulin E
Kluyveromyces
recombinant proteins
serum albumin
science & technology
life sciences & biomedicine
nutrition & dietetics
Summary Chicken serum albumin (CSA) is a hen's egg yolk allergen causing IgE-mediated allergy. The objective of this study was to produce a recombinant version of CSA and compare its IgE reactivity to natural CSA (nCSA). CSA was cloned and expressed as a soluble fraction in the yeast Kluyveromyces lactis (K. lactis) protein expression system. The gene encoding CSA was amplified with a C-terminal hemagglutinin epitope tag by polymerase chain reaction (PCR) and cloned into the pKLAC2 expression vector prior to transforming into K. lactis. Recombinant CSA (rCSA) was purified by immunoprecipitation. Twenty-one patients allergic to hen's egg white were examined for sensitisation against nCSA. 38% of patients were found to be sensitised to CSA based on Western immunoassay. Immunoglobulin E (IgE) binding capacity of rCSA and nCSA was analysed by ELISA using sera from patients sensitised to CSA. Levels of IgE-binding were similar for both the recombinant and the natural CSA, indicating the existence of similar epitopes. rCSA produced in this study is a potential candidate to be used in component-resolved diagnosis (CRD) of egg yolk allergy. The usefulness of rCSA in CRD of egg yolk allergy warrants further characterisation using sera from patients with allergy to hen's egg yolk in future studies.
Language eng
DOI 10.3390/nu10060757
Field of Research 1111 Nutrition And Dietetics
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2018, the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30111726

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.