Openly accessible

Intrastructural help: harnessing T helper cells induced by licensed vaccines for improvement of HIV env antibody responses to virus-like particle vaccines

Elsayed, Hassan, Nabi, Ghulam, McKinstry, William J., Khoo, Keith K., Mak, Johnson, Salazar, Andres M., Tenbusch, Matthias, Temchura, Vladimir and Überla, Klaus 2018, Intrastructural help: harnessing T helper cells induced by licensed vaccines for improvement of HIV env antibody responses to virus-like particle vaccines, Journal of virology, vol. 92, no. 14, pp. 1-15, doi: 10.1128/JVI.00141-18.

Attached Files
Name Description MIMEType Size Downloads
khoo-intrastructuralhelp-2018.pdf Published version application/pdf 2.10MB 4

Title Intrastructural help: harnessing T helper cells induced by licensed vaccines for improvement of HIV env antibody responses to virus-like particle vaccines
Author(s) Elsayed, Hassan
Nabi, Ghulam
McKinstry, William J.
Khoo, Keith K.
Mak, JohnsonORCID iD for Mak, Johnson orcid.org/0000-0002-5229-5707
Salazar, Andres M.
Tenbusch, Matthias
Temchura, Vladimir
Überla, Klaus
Journal name Journal of virology
Volume number 92
Issue number 14
Article ID e00141-18
Start page 1
End page 15
Total pages 15
Publisher American Society for Microbiology
Place of publication Washington, D.C.
Publication date 2018-07
ISSN 1098-5514
Keyword(s) Env
HIV
IgG subtype
T helper cells
VLP
antibody
intrastructural help
vaccine
AIDS Vaccines
Animals
Antibody Formation
HEK293 Cells
HIV Antibodies
HIV Infections
HIV-1
Humans
Immunization, Secondary
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
T-Lymphocytes, Helper-Inducer
Vaccination
Vaccines, Virus-Like Particle
env Gene Products, Human Immunodeficiency Virus
Science & Technology
Life Sciences & Biomedicine
Virology
ENVELOPE GLYCOPROTEINS
PROTECTIVE IMMUNITY
B-CELLS
PROTEIN
PURIFICATION
IMMUNIZATION
EPITOPES
PEPTIDE
Summary Induction of persistent antibody responses by vaccination is generally thought to depend on efficient help by T follicular helper cells. Since the T helper cell response to HIV Env may not be optimal, we explored the possibility of improving the HIV Env antibody response to virus-like particle (VLP) vaccines by recruiting T helper cells induced by commonly used licensed vaccines to provide help for Env-specific B cells. B cells specific for the surface protein of a VLP can internalize the entire VLP and thus present peptides derived from the surface and core proteins on their major histocompatibility complex class II (MHC-II) molecules. This allows T helper cells specific for the core protein to provide intrastructural help for B cells recognizing the surface protein. Consistently, priming mice with an adjuvanted Gag protein vaccine enhanced the HIV Env antibody response to subsequent booster immunizations with HIV VLPs. To harness T helper cells induced by the licensed Tetanolpur vaccines, HIV VLPs that contained T helper cell epitopes of tetanus toxoid were generated. Tetanol-immunized mice raised stronger antibody responses to immunizations with VLPs containing tetanus toxoid T helper cell epitopes but not to VLPs lacking these epitopes. Depending on the priming immunization, the IgG subtype response to HIV Env after the VLP immunization could also be modified. Thus, harnessing T helper cells induced by other vaccines appears to be a promising approach to improve the HIV Env antibody response to VLP vaccines.
Language eng
DOI 10.1128/JVI.00141-18
Field of Research 06 Biological Sciences
07 Agricultural And Veterinary Sciences
11 Medical And Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2018 Elsayed et al.
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30111874

Document type: Journal Article
Collections: School of Medicine
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 14 Abstract Views, 5 File Downloads  -  Detailed Statistics
Created: Fri, 27 Jul 2018, 12:41:51 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.