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Protective effects of a lipid extract from hard-shelled mussel (Mytilus coruscus) on intestinal integrity after lipopolysaccharide challenge in mice

Wan, Yi, Fu, Yuanqing, Wang, Fenglei, Sinclair, Andrew J. and Li, Duo 2018, Protective effects of a lipid extract from hard-shelled mussel (Mytilus coruscus) on intestinal integrity after lipopolysaccharide challenge in mice, Nutrients, vol. 10, no. 7, doi: 10.3390/nu10070860.

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Title Protective effects of a lipid extract from hard-shelled mussel (Mytilus coruscus) on intestinal integrity after lipopolysaccharide challenge in mice
Formatted title Protective effects of a lipid extract from hard-shelled mussel (Mytilus coruscus) on intestinal integrity after lipopolysaccharide challenge in mice
Author(s) Wan, Yi
Fu, Yuanqing
Wang, Fenglei
Sinclair, Andrew J.
Li, Duo
Journal name Nutrients
Volume number 10
Issue number 7
Total pages 16
Publisher MDPI AG
Place of publication Basel, Switzerland
Publication date 2018-07-03
ISSN 2072-6643
Keyword(s) Mytilus coruscus
TLR-4 signaling pathway
hard-shelled mussel
intestinal integrity
lipopolysaccharide
Summary This study investigated the protective effects of a lipid extract from hard-shelled mussel (HMLE) on intestinal integrity and the underlying mechanisms after a lipopolysaccharide (LPS) challenge in mice by using a 3 × 2 factorial design. Mice received olive oil, fish oil, and HMLE (n = 12 per group) by using gastric gavage for six weeks, respectively. Then half the mice in each group was injected intraperitoneally with LPS and the other half with phosphate buffered saline. Four hours after injection, mice were sacrificed and samples were collected. n-3 PUFAs were significantly enriched in erythrocytes following fish oil and HMLE supplementation. Both fish oil and HMLE improved intestinal morphology by restoring the ileac villus height and barrier function, which is indicated by decreased colonic myeloperoxidase activity and increased diamine oxidase activity as well as enhanced mRNA expression of intestinal tight junction proteins known as occludin and claudin-1 when compared with olive oil. In addition, both fish oil and HMLE increased colon production and the expression of anti-inflammatory cytokine, IL-10, while they inhibited the abnormal production and expression of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6 relative to the olive oil. Lastly, in comparison with olive oil, both fish oil and HMLE downregulated the TLR-4 signaling pathway by reducing the expression of two key molecules in this pathway, which are called TLR-4 and MyD88. These results suggest that HMLE had a protective effect on intestinal integrity after the LPS challenge, which was equivalent to that of fish oil. This effect might be associated with the regulation of inflammatory mediators and the inhibition of the TLR-4 signaling pathway.
Language eng
DOI 10.3390/nu10070860
Field of Research 1111 Nutrition And Dietetics
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2018, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30111893

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.