The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein Macnaughton, Lindsay S., Wardle, Sophie L., Witard, Oliver C., McGlory, Chris, Hamilton, D. Lee, Jeromson, Stewart, Lawrence, Clare E., Wallis, Gareth A. and Tipton, Kevin D. 2016, The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein, Physiological reports, vol. 4, no. 15, doi: 10.14814/phy2.12893. Attached Files Name Description MIMEType Size Downloads hamilton-responseofmuscle-2016.pdf Published version application/pdf 889.45KB 70 Title The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein Author(s) Macnaughton, Lindsay S. Wardle, Sophie L. Witard, Oliver C. McGlory, Chris Hamilton, D. Lee Jeromson, Stewart Lawrence, Clare E. Wallis, Gareth A. Tipton, Kevin D. Journal name Physiological reports Volume number 4 Issue number 15 Article ID e12893 Total pages 13 Publisher Wiley Place of publication Bethesda, Md. Publication date 2016-08 ISSN 2051-817X Keyword(s) Fractional synthesis rate intracellular signaling proteins lean body mass protein dose‐response Adult Amino Acids Body Weights and Measures Exercise Humans Male Muscle Proteins Muscle, Skeletal Myofibrils Phenylalanine Urea Whey Proteins Young Adult Summary The currently accepted amount of protein required to achieve maximal stimulation of myofibrillar protein synthesis (MPS) following resistance exercise is 20-25 g. However, the influence of lean body mass (LBM) on the response of MPS to protein ingestion is unclear. Our aim was to assess the influence of LBM, both total and the amount activated during exercise, on the maximal response of MPS to ingestion of 20 or 40 g of whey protein following a bout of whole-body resistance exercise. Resistance-trained males were assigned to a group with lower LBM (≤65 kg; LLBM n = 15) or higher LBM (≥70 kg; HLBM n = 15) and participated in two trials in random order. MPS was measured with the infusion of (13)C6-phenylalanine tracer and collection of muscle biopsies following ingestion of either 20 or 40 g protein during recovery from a single bout of whole-body resistance exercise. A similar response of MPS during exercise recovery was observed between LBM groups following protein ingestion (20 g - LLBM: 0.048 ± 0.018%·h(-1); HLBM: 0.051 ± 0.014%·h(-1); 40 g - LLBM: 0.059 ± 0.021%·h(-1); HLBM: 0.059 ± 0.012%·h(-1)). Overall (groups combined), MPS was stimulated to a greater extent following ingestion of 40 g (0.059 ± 0.020%·h(-1)) compared with 20 g (0.049 ± 0.020%·h(-1); P = 0.005) of protein. Our data indicate that ingestion of 40 g whey protein following whole-body resistance exercise stimulates a greater MPS response than 20 g in young resistance-trained men. However, with the current doses, the total amount of LBM does not seem to influence the response. Language eng DOI 10.14814/phy2.12893 HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2016, The Authors Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30112503 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Open Access Collection Related Links Link Description Connect to published version (open access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 16 Aug 2018, 11:43:02 EST Fri, 17 Aug 2018, 05:00:29 EST Tue, 23 Oct 2018, 05:02:22 EST Tue, 23 Oct 2018, 14:35:08 EST Tue, 23 Oct 2018, 14:38:24 EST Tue, 23 Oct 2018, 15:22:41 EST Wed, 24 Oct 2018, 08:35:10 EST Sat, 05 Sep 2020, 02:31:11 EST Filtered Full Citation counts:   Cited 56 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 155 Abstract Views, 72 File Downloads  -  Detailed Statistics Created: Thu, 16 Aug 2018, 11:43:02 EST

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