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Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men

McGlory, Chris, Wardle, Sophie L., Macnaughton, Lindsay S., Witard, Oliver C., Scott, Fraser, Dick, James, Bell, J. Gordon, Phillips, Stuart M., Galloway, Stuart D. R., Hamilton, D. Lee and Tipton, Kevin D. 2016, Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men, Physiological reports, vol. 4, no. 6, doi: 10.14814/phy2.12715.

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Title Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men
Author(s) McGlory, Chris
Wardle, Sophie L.
Macnaughton, Lindsay S.
Witard, Oliver C.
Scott, Fraser
Dick, James
Bell, J. Gordon
Phillips, Stuart M.
Galloway, Stuart D. R.
Hamilton, D. Lee
Tipton, Kevin D.
Journal name Physiological reports
Volume number 4
Issue number 6
Article ID e12715
Total pages 11
Publisher Wiley
Place of publication Bethesda, Md.
Publication date 2016-03
ISSN 2051-817X
Keyword(s) Fish oil
myofibrillar muscle protein synthesis
p70S6K1
resistance exercise
AMP-Activated Protein Kinases
Anabolic Agents
Biopsy
Dietary Proteins
Dietary Supplements
Fish Oils
Humans
Male
Muscle Proteins
Muscle, Skeletal
Myofibrils
Phospholipids
Phosphorylation
Protein Biosynthesis
Proto-Oncogene Proteins c-akt
Resistance Training
Ribosomal Protein S6 Kinases, 70-kDa
Scotland
Signal Transduction
Time Factors
Treatment Outcome
Young Adult
Summary Fish oil (FO) supplementation potentiates muscle protein synthesis (MPS) in response to a hyperaminoacidemic-hyperinsulinemic infusion. Whether FO supplementation potentiates MPS in response to protein ingestion or when protein ingestion is combined with resistance exercise (RE) remains unknown. In a randomized, parallel group design, 20 healthy males were randomized to receive 5 g/day of either FO or coconut oil control (CO) for 8 weeks. After supplementation, participants performed a bout of unilateral RE followed by ingestion of 30 g of whey protein. Skeletal muscle biopsies were obtained before and after supplementation for assessment of muscle lipid composition and relevant protein kinase activities. Infusion of L-[ring-(13)C6] phenylalanine was used to measure basal myofibrillar MP Sat rest (REST), in a nonexercised leg following protein ingestion (FED) and following RE and protein ingestion (FEDEX).MPS was significantly elevated above REST during FEDEX in both the FO and CO groups, but there was no effect of supplementation. There was a significant increase in MPS in both groups above REST during FED but no effect of supplementation. Supplementation significantly decreased pan PKB activity at RESTin the FO group but not the CO group. There was a significant increase from REST at post-RE for PKB and AMPKα2 activity in the CO group but not in the FO group. In FEDEX, there was a significant increase in p70S6K1 activity from REST at 3 h in the CO group only. These data highlight that 8 weeks of FO supplementation alters kinase signaling activity in response to RE plus protein ingestion without influencing MPS.
Language eng
DOI 10.14814/phy2.12715
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30112685

Document type: Journal Article
Collections: School of Exercise and Nutrition Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.