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RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells

Rodd, Annabelle L., Ververis, Katherine, Sayakkarage, Dheeshana, Khan, Abdul W., Rafehi, Haloom, Ziemann, Mark, Loveridge, Shanon J., Lazarus, Ross, Kerr, Caroline, Lockett, Trevor, El-Osta, Assam, Karagiannis, Tom C. and Bennett, Louise E. 2015, RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells, Food & function, vol. 6, no. 8, pp. 2507-2524, doi: 10.1039/c5fo00467e.

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Title RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells
Formatted title RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells
Author(s) Rodd, Annabelle L.
Ververis, Katherine
Sayakkarage, Dheeshana
Khan, Abdul W.
Rafehi, Haloom
Ziemann, MarkORCID iD for Ziemann, Mark orcid.org/0000-0002-7688-6974
Loveridge, Shanon J.
Lazarus, Ross
Kerr, Caroline
Lockett, Trevor
El-Osta, Assam
Karagiannis, Tom C.
Bennett, Louise E.
Journal name Food & function
Volume number 6
Issue number 8
Start page 2507
End page 2524
Total pages 18
Publisher Royal Society of Chemistry
Place of publication Cambridge, England
Publication date 2015-08
ISSN 2042-650X
Keyword(s) Animals
Apoptosis
Cell Cycle
Cell Differentiation
Colorectal Neoplasms
DNA Breaks, Double-Stranded
HT29 Cells
Humans
Laurus
Molecular Weight
Plant Extracts
Plant Leaves
Reactive Oxygen Species
Sequence Analysis, RNA
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Food Science & Technology
COLON-CANCER CELLS
ACTIVATING TRANSCRIPTION FACTOR-3
E-CADHERIN EXPRESSION
GROWTH IN-VITRO
COLORECTAL-CANCER
DNA-DAMAGE
GENE-EXPRESSION
CARCINOMA-CELLS
TUMOR-CELLS
DIETARY CONSTITUENTS
Summary Anti-proliferative and pro-apoptotic effects of Bay leaf (Laurus nobilis) in mammalian cancer and HT-29 adenocarcinoma cells have been previously attributed to effects of polyphenolic and essential oil chemical species. Recently, we demonstrated differentiated growth-regulating effects of high (HFBL) versus low molecular mass (LFBL) aqueous fractions of bay leaf and now confirm by comparative effects on gene expression, that HFBL and LFBL suppress HT-29 growth by distinct mechanisms. Induction of intra-cellular lesions including DNA strand breakage by extra-cellular HFBL, invoked the hypothesis that iron-mediated reactive oxygen species with capacity to penetrate cell membrane, were responsible for HFBL-mediated effects, supported by equivalent effects of HFBL in combination with γ radiation. Activities of HFBL and LFBL were interpreted to reflect differentiated responses to iron-mediated reactive oxygen species (ROS), occurring either outside or inside cells. In the presence of LFBL, apoptotic death was relatively delayed compared with HFBL. ROS production by LFBL mediated p53-dependent apoptosis and recovery was suppressed by promoting G1/S phase arrest and failure of cellular tight junctions. In comparison, intra-cellular anti-oxidant protection exerted by LFBL was absent for extra-cellular HFBL (likely polysaccharide-rich), which potentiated more rapid apoptosis by producing DNA double strand breaks. Differentiated effects on expression of genes regulating ROS defense and chromatic condensation by LFBL versus HFBL, were observed. The results support ferrous iron in cell culture systems and potentially in vivo, can invoke different extra-cellular versus intra-cellular ROS-mediated chemistries, that may be regulated by exogenous, including dietary species.
Language eng
DOI 10.1039/c5fo00467e
Field of Research 0908 Food Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Royal Society of Chemistry
Free to Read? Yes
Use Rights Creative Commons Attribution non-commercial licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30112914

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.