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Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family

Hove, Runyararo M., Ziemann, Mark and Bhave, Mrinal 2015, Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family, PLOS One, vol. 10, no. 6, pp. 1-21, doi: 10.1371/journal.pone.0128025.

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Title Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family
Formatted title Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family
Author(s) Hove, Runyararo M.
Ziemann, MarkORCID iD for Ziemann, Mark orcid.org/0000-0002-7688-6974
Bhave, Mrinal
Journal name PLOS One
Volume number 10
Issue number 6
Article ID e0128025
Start page 1
End page 21
Total pages 21
Publisher Public Library of Science
Place of publication San Francisco, Calif
Publication date 2015-06
ISSN 1932-6203
Keyword(s) Aquaporins
Exons
Gene Expression Regulation, Plant
Genes, Plant
Hordeum
Introns
Molecular Sequence Annotation
Multigene Family
Oligonucleotide Array Sequence Analysis
Phylogeny
Plant Proteins
Protein Transport
Salinity
Stress, Physiological
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
PLASMA-MEMBRANE AQUAPORINS
MAJOR INTRINSIC PROTEINS
PHYSCOMITRELLA-PATENS
SILICON TRANSPORTER
WATER PERMEABILITY
PLANT AQUAPORINS
PIP AQUAPORINS
RICE
STRESS
ROOTS
Summary Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, and play critical roles in plant-water relations, dehydration stress responses and crop productivity. However, limited data are available as yet on the contributions of these proteins to the physiology of the major crop barley (Hordeum vulgare). The present work reports the identification and expression analysis of the barley MIP family. A comprehensive search of publicly available leaf mRNA-seq data, draft barley genome data, GenBank transcripts and sixteen new annotations together revealed that the barley MIP family is comprised of at least forty AQPs. Alternative splicing events were likely in two plasma membrane intrinsic protein (PIP) AQPs. Analyses of the AQP signature sequences and specificity determining positions indicated a potential of several putative AQP isoforms to transport non-aqua substrates including physiological important substrates, and respond to abiotic stresses. Analysis of our publicly available leaf mRNA-seq data identified notable differential expression of HvPIP1;2 and HvTIP4;1 under salt stress. Analyses of other gene expression resources also confirmed isoform-specific responses in different tissues and/or in response to salinity, as well as some potentially inter-cultivar differences. The work reports systematic and comprehensive analysis of most, if not all, barley AQP genes, their sequences, expression patterns in different tissues, potential transport and stress response functions, and a strong framework for selection and/or development of stress tolerant barley varieties. In addition, the barley data would be highly valuable for genetic studies of the evolutionarily closely related wheat (Triticum aestivum L.).
Language eng
DOI 10.1371/journal.pone.0128025
Field of Research MD Multidisciplinary
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Hove et al.
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30112916

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.