Set7 mediated interactions regulate transcriptional networks in embryonic stem cells

Tuano, Natasha K, Okabe, Jun, Ziemann, Mark, Cooper, Mark E and El-Osta, Assam 2016, Set7 mediated interactions regulate transcriptional networks in embryonic stem cells, Nucleic acids research, vol. 44, no. 19, pp. 9206-9217, doi: 10.1093/nar/gkw621.

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Title Set7 mediated interactions regulate transcriptional networks in embryonic stem cells
Author(s) Tuano, Natasha K
Okabe, Jun
Ziemann, MarkORCID iD for Ziemann, Mark orcid.org/0000-0002-7688-6974
Cooper, Mark E
El-Osta, Assam
Journal name Nucleic acids research
Volume number 44
Issue number 19
Start page 9206
End page 9217
Total pages 12
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 2016-11-02
ISSN 0305-1048
1362-4962
Keyword(s) ataxin-1
biomarkers
cell differentiation
cell line
cluster analysis
embryonic stem cells
enzyme activation
gene expression profiling
gene expression regulation
gene regulatory networks
histone-lysine N-methyltransferase
mice
models, biological
octamer transcription factor-3
phenotype
SOXB1 transcription factors
transcription, genetic
science & technology
life sciences & biomedicine
biochemistry & molecular biology
Summary Histone methylation by lysine methyltransferase enzymes regulate the expression of genes implicated in lineage specificity and cellular differentiation. While it is known that Set7 catalyzes mono-methylation of histone and non-histone proteins, the functional importance of this enzyme in stem cell differentiation remains poorly understood. We show Set7 expression is increased during mouse embryonic stem cell (mESC) differentiation and is regulated by the pluripotency factors, Oct4 and Sox2. Transcriptional network analyses reveal smooth muscle (SM) associated genes are subject to Set7-mediated regulation. Furthermore, pharmacological inhibition of Set7 activity confirms this regulation. We observe Set7-mediated modification of serum response factor (SRF) and mono-methylation of histone H4 lysine 4 (H3K4me1) regulate gene expression. We conclude the broad substrate specificity of Set7 serves to control key transcriptional networks in embryonic stem cells.
Language eng
DOI 10.1093/nar/gkw621
Field of Research 05 Environmental Sciences
06 Biological Sciences
08 Information And Computing Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30113075

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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