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Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites

Onyango, Maria G., Beebe, Nigel W., Gopurenko, David, Bellis, Glenn, Nicholas, Adrian, Ogugo, Moses, Djikeng, Appolinaire, Kemp, Steve, Walker, Peter J. and Duchemin, Jean-Bernard 2015, Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites, Veterinary research, vol. 46, pp. 1-9, doi: 10.1186/s13567-015-0250-8.

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Title Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites
Formatted title Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites
Author(s) Onyango, Maria G.
Beebe, Nigel W.
Gopurenko, David
Bellis, Glenn
Nicholas, Adrian
Ogugo, Moses
Djikeng, Appolinaire
Kemp, Steve
Walker, Peter J.
Duchemin, Jean-Bernard
Journal name Veterinary research
Volume number 46
Article ID 108
Start page 1
End page 9
Total pages 9
Publisher BMC
Place of publication Berlin, Germany
Publication date 2015-09-25
ISSN 1297-9716
Keyword(s) Animals
Australia
Bluetongue virus
Ceratopogonidae
DNA
Genetic Variation
Genetics, Population
Insect Vectors
Microsatellite Repeats
Papua New Guinea
Timor-Leste
Science & Technology
Life Sciences & Biomedicine
Veterinary Sciences
AUSTRALASIAN REGION
ANOPHELES-GAMBIAE
GENOTYPING ERRORS
BITING MIDGES
PRIMER DESIGN
NULL ALLELES
SOFTWARE
DISTANCE
INDIVIDUALS
INCURSIONS
Summary Bluetongue virus (BTV) is a major pathogen of ruminants that is transmitted by biting midges (Culicoides spp.). Australian BTV serotypes have origins in Asia and are distributed across the continent into two distinct episystems, one in the north and another in the east. Culicoides brevitarsis is the major vector of BTV in Australia and is distributed across the entire geographic range of the virus. Here, we describe the isolation and use of DNA microsatellites and gauge their ability to determine population genetic connectivity of C. brevitarsis within Australia and with countries to the north. Eleven DNA microsatellite markers were isolated using a novel genomic enrichment method and identified as useful for genetic analyses of sampled populations in Australia, northern Papua New Guinea (PNG) and Timor-Leste. Significant (P < 0.05) population genetic subdivision was observed between all paired regions, though the highest levels of genetic sub-division involved pair-wise tests with PNG (PNG vs. Australia (FST = 0.120) and PNG vs. Timor-Leste (FST = 0.095)). Analysis of multi-locus allelic distributions using STRUCTURE identified a most probable two-cluster population model, which separated PNG specimens from a cluster containing specimens from Timor-Leste and Australia. The source of incursions of this species in Australia is more likely to be Timor-Leste than PNG. Future incursions of BTV positive C. brevitarsis into Australia may be genetically identified to their source populations using these microsatellite loci. The vector’s panmictic genetic structure within Australia cannot explain the differential geographic distribution of BTV serotypes.
Language eng
DOI 10.1186/s13567-015-0250-8
Field of Research 0605 Microbiology
0707 Veterinary Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30113363

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.