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Dual functional ultrafiltration membranes with enzymatic digestion and thermo-responsivity for protein self-cleaning

Vanangamudi, Anbharasi, Dumee, Ludovic F, Duke, Mikel C and Yang, Xing 2018, Dual functional ultrafiltration membranes with enzymatic digestion and thermo-responsivity for protein self-cleaning, Membranes, vol. 8, no. 3, pp. 1-15, doi: 10.3390/membranes8030085.

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Title Dual functional ultrafiltration membranes with enzymatic digestion and thermo-responsivity for protein self-cleaning
Author(s) Vanangamudi, Anbharasi
Dumee, Ludovic FORCID iD for Dumee, Ludovic F orcid.org/0000-0002-0264-4024
Duke, Mikel C
Yang, Xing
Journal name Membranes
Volume number 8
Issue number 3
Start page 1
End page 15
Total pages 15
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2018-09-19
ISSN 2077-0375
Keyword(s) thermo-responsive
ultrafiltration
enzymes
self-cleaning
nanofibers
Summary Controlling surface⁻protein interaction during wastewater treatment is the key motivation for developing functionally modified membranes. A new biocatalytic thermo-responsive poly vinylidene fluoride (PVDF)/nylon-6,6/poly(N-isopropylacrylamide)(PNIPAAm) ultrafiltration membrane was fabricated to achieve dual functionality of protein-digestion and thermo-responsive self-cleaning. The PVDF/nylon-6,6/PNIPAAm composite membranes were constructed by integrating a hydrophobic PVDF cast layer and hydrophilic nylon-6,6/PNIPAAm nanofiber layer on to which trypsin was covalently immobilized. The enzyme immobilization density on the membrane surface decreased with increasing PNIPAAm concentration, due to the decreased number of amine functional sites. An ultrafiltration study was performed using the synthetic model solution containing BSA/NaCl/CaCl2, where the PNIPAAm containing biocatalytic membranes demonstrated a combined effect of enzymatic and thermo-switchable self-cleaning. The membrane without PNIPAAm revealed superior fouling resistance and self-cleaning with an RPD of 22%, compared to membranes with 2 and 4 wt % PNIPAAm with 26% and 33% RPD, respectively, after an intermediate temperature cleaning at 50 °C, indicating that higher enzyme density offers more efficient self-cleaning than the combined effect of enzyme and PNIPAAm at low concentration. The conformational volume phase transition of PNIPAAm did not affect the stability of immobilized trypsin on membrane surfaces. Such novel surface engineering design offer a promising route to mitigate surface⁻protein contamination in wastewater applications.
Language eng
DOI 10.3390/membranes8030085
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2018, the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30114006

Document type: Journal Article
Collections: Institute for Frontier Materials
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.