The ontogeny of hepatic growth hormone receptor and insulin-like growth factor I gene expression in the sheep fetus during late gestation: developmental regulation by cortisol

Li, J., Owens, J. A., Owens, P. C., Saunders, J. C., Fowden, A. L. and Gilmour, R. S. 1996, The ontogeny of hepatic growth hormone receptor and insulin-like growth factor I gene expression in the sheep fetus during late gestation: developmental regulation by cortisol, Endocrinology, vol. 137, no. 5, pp. 1650-1657, doi: 10.1210/endo.137.5.8612497.

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Title The ontogeny of hepatic growth hormone receptor and insulin-like growth factor I gene expression in the sheep fetus during late gestation: developmental regulation by cortisol
Author(s) Li, J.
Owens, J. A.ORCID iD for Owens, J. A. orcid.org/0000-0002-7498-1353
Owens, P. C.
Saunders, J. C.
Fowden, A. L.
Gilmour, R. S.
Journal name Endocrinology
Volume number 137
Issue number 5
Start page 1650
End page 1657
Total pages 8
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 1996-05
ISSN 0013-7227
Keyword(s) Adrenalectomy
Animals
Base Sequence
Female
Fetal Blood
Gene Expression Regulation, Developmental
Gestational Age
Hydrocortisone
Insulin-Like Growth Factor I
Liver
Molecular Sequence Data
RNA, Messenger
Receptors, Somatotropin
Sheep
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
MESSENGER-RIBONUCLEIC-ACID
IGF-I
FETAL TISSUES
RNA
BINDING
LAMB
HYPOPHYSECTOMY
PARTURITION
BIRTH
FALL
Summary The effects of cortisol on hepatic GH receptor and insulin-like growth factor-I (IGF-I) gene expression were investigated in sheep fetuses during late gestation and after experimental manipulation of plasma cortisol levels by fetal adrenalectomy and exogenous infusion of cortisol. Hepatic GH receptor and IGF-I messenger RNA (mRNA) levels increased with increasing gestational age in parallel with the normal rise in fetal cortisol levels toward term (145 +/- 2 days). These increases in mRNA abundance toward term were prevented when the prepartum cortisol surge was abolished by fetal adrenalectomy and were stimulated prematurely in fetuses younger than 130 days by exogenous infusion of cortisol. Both the class 1 and class 2 transcripts of the IGF-I gene were increased when cortisol levels were elevated either endogenously or exogenously. However, there were no significant changes in fetal plasma IGF-I levels either with increasing gestational age or in response to experimental manipulation of the fetal cortisol level. When the data from all the fetuses were combined irrespective of treatment or gestational age, there were significant positive correlations between the log plasma cortisol concentration in utero and the abundance of GH receptor and IGF-I mRNA in the fetal liver. There was also a significant inverse relationship between log plasma cortisol and the ratio of class 1 to class 2 transcript abundance in the fetal liver. These findings show that cortisol is a physiological regulator of hepatic GH receptor and IGF-I gene expression in fetal sheep during late gestation and indicate that it preferentially increases the class 2 transcript of the IGF-I gene. The prepartum cortisol surge therefore appears to have an important maturational role in initiating the perinatal switch from the fetal to adult modes of somatotrophic regulation.
Language eng
DOI 10.1210/endo.137.5.8612497
Field of Research 07 Agricultural And Veterinary Sciences
11 Medical And Health Sciences
06 Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1996, The Endocrmc Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30116690

Document type: Journal Article
Collection: Office of the Deputy Vice-Chancellor (Research)
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