Impact of restriction of placental and fetal growth on expression of 11ß-hydroxysteroid dehydrogenase type 1 and type 2 messenger ribonucleic acid in the liver, kidney, and adrenal of the sheep fetus

McMillen, I. C., Warnes, K. E., Adams, M. B., Robinson, J. S., Owens, J. A. and Coulter, C. L. 2000, Impact of restriction of placental and fetal growth on expression of 11ß-hydroxysteroid dehydrogenase type 1 and type 2 messenger ribonucleic acid in the liver, kidney, and adrenal of the sheep fetus, Endocrinology, vol. 141, no. 2, pp. 539-543, doi: 10.1210/endo.141.2.7338.

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Title Impact of restriction of placental and fetal growth on expression of 11ß-hydroxysteroid dehydrogenase type 1 and type 2 messenger ribonucleic acid in the liver, kidney, and adrenal of the sheep fetus
Author(s) McMillen, I. C.
Warnes, K. E.
Adams, M. B.
Robinson, J. S.
Owens, J. A.ORCID iD for Owens, J. A. orcid.org/0000-0002-7498-1353
Coulter, C. L.
Journal name Endocrinology
Volume number 141
Issue number 2
Start page 539
End page 543
Total pages 5
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 2000-02-01
ISSN 0013-7227
Keyword(s) 11-beta-Hydroxysteroid Dehydrogenase Type 1
Adrenal Glands
Animals
Body Weight
Embryonic and Fetal Development
Female
Fetal Growth Retardation
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gestational Age
Hydroxysteroid Dehydrogenases
Isoenzymes
Kidney
Liver
Placenta
Pregnancy
RNA, Messenger
Sheep
Transcription, Genetic
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
RECEPTOR GENE-EXPRESSION
CELLULAR-LOCALIZATION
ADULT HYPERTENSION
BLOOD-PRESSURE
LATE-GESTATION
BIRTH-WEIGHT
CORTISOL
PITUITARY
EXPOSURE
STEROIDOGENESIS
Summary We have investigated the effects of fetal growth restriction, induced by restriction of placental growth and function (PR), on 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD-1) and 11betaHSD-2 messenger RNA (mRNA) expression in fetal tissues in the sheep, using Northern blot analysis. Fetal liver, kidney, and adrenals were collected from normally grown fetuses at 90 days (n = 6), 125 days (n = 6), and 141-145 days (n = 7) and from PR fetuses at 141-145 days (n = 6). Expression of 11betaHSD-1 mRNA in the fetal liver increased significantly between 125 days (7.4+/-0.8) and 141-145 days gestation (27+/-5.3). There was also an approximately 2-fold increase in the ratio of 11betaHSD-1 mRNA/18S rRNA expression in the PR group (53.8+/-7.9) compared with that in control animals at 141-145 days gestation. There was a significant decrease in 11betaHSD-2 mRNA in fetal adrenals between 125 days (41.6+/-2.4) and 141-145 days (26.7+/-1.1) gestation, but there was no effect of PR on the expression of adrenal 11betaHSD-2 mRNA. 11betaHSD-2 mRNA expression in the fetal kidney increased between 90 days (16.8+/-1.7) and 141-145 days gestation (31.7+/-4.3), but there was no effect of PR on the levels of 11betaHSD-2 mRNA in the fetal kidney. In summary, 11betaHSD-2 mRNA is differentially regulated in the fetal adrenal and kidney in the sheep fetus during late gestation. There is also a specific increase in the expression of 11betaHSD-1 mRNA in the liver of growth-restricted fetuses in late gestation. This suggests that there is increased hepatic exposure to cortisol in the growth-restricted fetus, which may be important in the reprogramming of hepatic physiology that occurs after growth restriction in utero.
Language eng
DOI 10.1210/endo.141.2.7338
Field of Research 07 Agricultural And Veterinary Sciences
11 Medical And Health Sciences
06 Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2000, The Endocrine Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30116721

Document type: Journal Article
Collection: Office of the Deputy Vice-Chancellor Research
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