Intrafetal insulin-like growth factor-I infusion stimulates adrenal growth but not steroidogenesis in the sheep fetus during late gestation

Ross, J. T., McMillen, I. C., Lok, F., Thiel, A. G., Owens, J. A. and Coulter, C. L. 2007, Intrafetal insulin-like growth factor-I infusion stimulates adrenal growth but not steroidogenesis in the sheep fetus during late gestation, Endocrinology, vol. 148, no. 11, pp. 5424-5432, doi: 10.1210/en.2006-1573.

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Title Intrafetal insulin-like growth factor-I infusion stimulates adrenal growth but not steroidogenesis in the sheep fetus during late gestation
Author(s) Ross, J. T.
McMillen, I. C.
Lok, F.
Thiel, A. G.
Owens, J. A.ORCID iD for Owens, J. A. orcid.org/0000-0002-7498-1353
Coulter, C. L.
Journal name Endocrinology
Volume number 148
Issue number 11
Start page 5424
End page 5432
Total pages 9
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 2007-11-01
ISSN 0013-7227
Keyword(s) Adrenal Glands
Animals
Female
Fetal Blood
Fetal Development
Fetal Weight
Fetus
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gestational Age
Hydrocortisone
Insulin-Like Growth Factor I
Organ Size
Pregnancy
Sheep
Steroids
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
MESSENGER-RIBONUCLEIC-ACID
BOVINE CHROMAFFIN CELLS
IGF-BINDING-PROTEINS
FETAL SHEEP
CATECHOLAMINE SECRETION
FUNCTIONAL MATURATION
TYROSINE-HYDROXYLASE
ANGIOTENSIN-II
RNA LEVELS
PITUITARY
Summary We investigated the effects of an intrafetal infusion of IGF-I on adrenal growth and expression of the adrenal steroidogenic and catecholamine-synthetic enzyme mRNAs in the sheep fetus during late gestation. Fetal sheep were infused for 10 d with either IGF-I (26 microg/kg.h; n = 14) or saline (n = 10) between 120 and 130 d gestation, and adrenal glands were collected for morphological analysis and determination of the mRNA expression of steroidogenic and catecholamine-synthetic enzymes. Fetal body weight was not altered by IGF-I infusion; however, adrenal weight was significantly increased by 145% after IGF-I infusion. The density of cell nuclei within the fetal adrenal cortex (the zona glomerulosa and zona fasciculata), and within the adrenaline synthesizing zone of the adrenal medulla, was significantly less in the IGF-I-infused fetuses compared with the saline-infused group. Thus, based on cell-density measurements, there was a significant increase in cell size in the zona glomerulosa and zona fasciculata of the adrenal cortex and in the adrenaline-synthesizing zone of the adrenal medulla. There was no effect of IGF-I infusion on the adrenal mRNA expression of the steroidogenic or catecholamine-synthetic enzymes or on fetal plasma cortisol concentrations. In summary, infusion of IGF-I in late gestation resulted in a marked hypertrophy of the steroidogenic and adrenaline-containing cells of the fetal adrenal in the absence of changes in the mRNA levels of adrenal steroidogenic or catecholamine-synthetic enzymes or in fetal plasma cortisol concentrations. Thus, IGF-I infusion results in a dissociation of adrenal growth and function during late gestation.
Language eng
DOI 10.1210/en.2006-1573
Field of Research 07 Agricultural And Veterinary Sciences
11 Medical And Health Sciences
06 Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, The Endocrine Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30116773

Document type: Journal Article
Collection: Office of the Deputy Vice-Chancellor Research
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