Cross-fostering and improved lactation ameliorates deficits in endocrine pancreatic morphology in growth-restricted adult male rat offspring

Siebel, A. L., Gallo, T. C., Guan, T. C., Owens, J. A. and Wlodek M. E. 2010, Cross-fostering and improved lactation ameliorates deficits in endocrine pancreatic morphology in growth-restricted adult male rat offspring, Journal of developmental origins of health and disease, vol. 1, no. 4, pp. 234-244, doi: 10.1017/S2040174410000383.

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Title Cross-fostering and improved lactation ameliorates deficits in endocrine pancreatic morphology in growth-restricted adult male rat offspring
Author(s) Siebel, A. L.
Gallo, T. C.
Guan, T. C.
Owens, J. A.ORCID iD for Owens, J. A. orcid.org/0000-0002-7498-1353
Wlodek M. E.
Journal name Journal of developmental origins of health and disease
Volume number 1
Issue number 4
Start page 234
End page 244
Total pages 11
Publisher Cambridge University Press
Place of publication Cambridge, Eng.
Publication date 2010-08
ISSN 2040-1744
2040-1752
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
gene expression
nutrition
pancreatic beta-cell
BETA-CELL MASS
SPONTANEOUSLY HYPERTENSIVE-RAT
REDUCING LITTER SIZE
C-REACTIVE PROTEIN
UTEROPLACENTAL INSUFFICIENCY
INSULIN-RESISTANCE
FETAL-GROWTH
PLACENTAL RESTRICTION
MAMMARY FUNCTION
SKELETAL-MUSCLE
Summary Uteroplacental insufficiency and poor postnatal nutrition impair adult glucose tolerance and insulin secretion in male rat offspring, which can be partially ameliorated by improving postnatal nutrition. Uteroplacental insufficiency was induced in the WKY rat on day 18 of pregnancy (Restricted) compared to sham-operated Controls. Pups were then cross-fostered onto Control or Restricted mothers one day after birth resulting in: (Pup-on-Mother) Control-on-Control, Control-on-Restricted, Restricted-on-Control and Restricted-on-Restricted. Endocrine pancreatic morphology and markers of intrinsic β-cell function and glucose homeostasis were assessed in male offspring at 6 months. Pancreatic and hepatic gene expression was quantified at postnatal day 7 and 6 months. Restricted pups were born 10–15% lighter than Controls and remained lighter at 6 months. Relative islet and β-cell mass were 51–65% lower in Restricted-on-Restricted compared to Controls at 6 months. Non-fasting plasma C-reactive protein levels were also increased, suggestive of an inflammatory response. Overall, the average number of islets, small islets and proportion of β-cells per islet correlated positively with birth weight. Intrinsic β-cell function, estimated by insulin secretion relative to β-cell mass, was unaffected by Restriction, suggesting that the in vivo functional deficit was attributable to reduced mass, not function. Importantly, these deficits were ameliorated when lactational nutrition was normalized in Restricted-on-Control offspring, who also showed increased pancreatic Igf1r, Pdx1 and Vegf mRNA expression at 7 days compared to Control-on-Control and Restricted-on-Restricted. This highlights lactation as a critical period for intervention following prenatal restraint, whereby deficits in endocrine pancreatic mass and associated impaired in vivo insulin secretion can be ameliorated.
Language eng
DOI 10.1017/S2040174410000383
Field of Research 11 Medical And Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, Cambridge University Press and the International Society for Developmental Origins of Health and Disease
Persistent URL http://hdl.handle.net/10536/DRO/DU:30116781

Document type: Journal Article
Collection: Office of the Deputy Vice-Chancellor (Research)
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