Metabolomics and 16S rRNA sequencing of human colorectal cancers and adjacent mucosa

Loke, Mun Fai, Chua, Eng Guan, Gan, Han Ming, Thulasi, Kumar, Wanyiri, Jane W., Thevambiga, Iyadorai, Goh, Khean Lee, Wong, Won Fen and Vadivelu, Jamuna 2018, Metabolomics and 16S rRNA sequencing of human colorectal cancers and adjacent mucosa, PLoS one, vol. 13, no. 12, doi: 10.1371/journal.pone.0208584.

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Title Metabolomics and 16S rRNA sequencing of human colorectal cancers and adjacent mucosa
Author(s) Loke, Mun Fai
Chua, Eng Guan
Gan, Han MingORCID iD for Gan, Han Ming
Thulasi, Kumar
Wanyiri, Jane W.
Thevambiga, Iyadorai
Goh, Khean Lee
Wong, Won Fen
Vadivelu, Jamuna
Journal name PLoS one
Volume number 13
Issue number 12
Article ID e0208584
Total pages 20
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2018-12-21
ISSN 1932-6203
Summary Colorectal cancer (CRC) is ranked the third most common cancer in human worldwide. However, the exact mechanisms of CRC are not well established. Furthermore, there may be differences between mechanisms of CRC in the Asian and in the Western populations. In the present study, we utilized a liquid chromatography-mass spectrometry (LC-MS) metabolomic approach supported by the 16S rRNA next-generation sequencing to investigate the functional and taxonomical differences between paired tumor and unaffected (normal) surgical biopsy tissues from 17 Malaysian patients. Metabolomic differences associated with steroid biosynthesis, terpenoid biosynthesis and bile metabolism could be attributed to microbiome differences between normal and tumor sites. The relative abundances of Anaerotruncus, Intestinimonas and Oscillibacter displayed significant relationships with both steroid biosynthesis and terpenoid and triterpenoid biosynthesis pathways. Metabolites involved in serotonergic synapse/ tryptophan metabolism (Serotonin and 5-Hydroxy-3-indoleacetic acid [5-HIAA]) were only detected in normal tissue samples. On the other hand, S-Adenosyl-L-homocysteine (SAH), a metabolite involves in methionine metabolism and methylation, was frequently increased in tumor relative to normal tissues. In conclusion, this study suggests that local microbiome dysbiosis may contribute to functional changes at the cancer sites. Results from the current study also contributed to the list of metabolites that are found to differ between normal and tumor sites in CRC and supported our quest for understanding the mechanisms of carcinogenesis.
Language eng
DOI 10.1371/journal.pone.0208584
Field of Research MD Multidisciplinary
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2018, Loke et al.
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