mVps34 is activated following high-resistance contractions

MacKenzie, Matthew G., Hamilton, D. Lee, Murray, James T., Taylor, Peter M. and Baar, Keith 2009, mVps34 is activated following high-resistance contractions, The journal of physiology, vol. 587, no. 1, pp. 253-260, doi: 10.1113/jphysiol.2008.159830.

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Title mVps34 is activated following high-resistance contractions
Author(s) MacKenzie, Matthew G.
Hamilton, D. LeeORCID iD for Hamilton, D. Lee orcid.org/0000-0002-5620-4788
Murray, James T.
Taylor, Peter M.
Baar, Keith
Journal name The journal of physiology
Volume number 587
Issue number 1
Start page 253
End page 260
Total pages 8
Publisher Wiley
Place of publication Chichester, Eng.
Publication date 2009-01-15
ISSN 0022-3751
1469-7793
Keyword(s) Animals
Cell Line
Enzyme Activation
Female
Hypertrophy
Leucine
Mice
Muscle Contraction
Muscle, Skeletal
Phosphatidylinositol 3-Kinases
Physical Exertion
Rats
Rats, Wistar
Resistance Training
Ribosomal Protein S6 Kinases
Signal Transduction
Stress, Mechanical
Transcription Factors
Science & Technology
Life Sciences & Biomedicine
Neurosciences
Physiology
Neurosciences & Neurology
SKELETAL-MUSCLE HYPERTROPHY
RAPAMYCIN-DEPENDENT MANNER
EXERCISE-INDUCED INCREASE
AMINO-ACID-TRANSPORT
PROTEIN-SYNTHESIS
SACCHAROMYCES-CEREVISIAE
MAMMALIAN TARGET
KINASE
GROWTH
3-KINASE
Summary Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 +/- 112.08%, 3 h = 124.7 +/- 15.96% and 6 h = 129.2 +/- 0%) and mVps34 (3 h = 68.8 +/- 15.1% and 6 h = 36.0 +/- 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 +/- 2.29% and 3 h = 47.0 +/- 6.65%; mVps34 3 h = 24.5 +/- 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.
Language eng
DOI 10.1113/jphysiol.2008.159830
Field of Research 06 Biological Sciences
11 Medical And Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30117935

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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