Permissive transcriptional activity at the centromere through pockets of DNA hypomethylation

Wong, Nicholas C, Wong, Lee H, Quach, Julie M, Canham, Paul, Craig, Jeffrey M, Song, Jenny Z, Clark, Susan J and Choo, KH Andy 2006, Permissive transcriptional activity at the centromere through pockets of DNA hypomethylation, PLoS genetics, vol. 2, no. 2, pp. 0172-0186, doi: 10.1371/journal.pgen.0020017.

Attached Files
Name Description MIMEType Size Downloads

Title Permissive transcriptional activity at the centromere through pockets of DNA hypomethylation
Author(s) Wong, Nicholas C
Wong, Lee H
Quach, Julie M
Canham, Paul
Craig, Jeffrey MORCID iD for Craig, Jeffrey M
Song, Jenny Z
Clark, Susan J
Choo, KH Andy
Journal name PLoS genetics
Volume number 2
Issue number 2
Article ID e17
Start page 0172
End page 0186
Total pages 15
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2006-02
ISSN 1553-7390
Keyword(s) Animals
CHO Cells
CpG Islands
DNA Methylation
Transcription, Genetic
Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
Summary DNA methylation is a hallmark of transcriptional silencing, yet transcription has been reported at the centromere. To address this apparent paradox, we employed a fully sequence-defined ectopic human centromere (or neocentromere) to investigate the relationship between DNA methylation and transcription. We used sodium bisulfite PCR and sequencing to determine the methylation status of 2,041 CpG dinucleotides distributed across a 6.76-Mbp chromosomal region containing a neocentromere. These CpG dinucleotides were associated with conventional and nonconventional CpG islands. We found an overall hypermethylation of the neocentric DNA at nonconventional CpG islands that we designated as CpG islets and CpG orphans. The observed hypermethylation was consistent with the presence of a presumed transcriptionally silent chromatin state at the neocentromere. Within this neocentric chromatin, specific sites of active transcription and the centromeric chromatin boundary are defined by DNA hypomethylation. Our data demonstrate, for the first time to our knowledge, a correlation between DNA methylation and centromere formation in mammals, and that transcription and "chromatin-boundary activity" are permissible at the centromere through the selective hypomethylation of pockets of sequences without compromising the overall silent chromatin state and function of the centromere.
Language eng
DOI 10.1371/journal.pgen.0020017
Field of Research 0604 Genetics
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Wong et al.
Persistent URL

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Checking
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 53 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 53 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Fri, 01 Mar 2019, 10:18:34 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact