Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns

Craig, Jeffrey M, Earle, Elizabeth, Canham, Paul, Wong, Lee H, Anderson, Melissa and Choo, KH Andy 2003, Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns, Human molecular genetics, vol. 12, no. 23, pp. 3109-3121, doi: 10.1093/hmg/ddg330.

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Title Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns
Author(s) Craig, Jeffrey MORCID iD for Craig, Jeffrey M orcid.org/0000-0003-3979-7849
Earle, Elizabeth
Canham, Paul
Wong, Lee H
Anderson, Melissa
Choo, KH Andy
Journal name Human molecular genetics
Volume number 12
Issue number 23
Start page 3109
End page 3121
Total pages 13
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 2003-12-01
ISSN 0964-6906
Keyword(s) Animals
Cell Line
Centromere
Chromatin
Chromosomal Proteins, Non-Histone
Fluorescent Antibody Technique
Humans
Kinetochores
Metaphase
Mice
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Genetics & Heredity
Summary We have examined the metaphase chromosomal localization of 15 proteins that have previously been described as involved in mammalian chromatin modification and/or transcriptional modulation. Immunofluorescence data indicate that all the proteins localize to human and mouse centromeres, a neocentromere, and the active centromere of a dicentric chromosome, with six of these proteins (Sin3A, PCAF, MYST, MBD2, ORC2, P300/CBP) being demonstrated at mammalian centromeres for the first time. Most of these proteins fall into two distinct chromosomal distribution patterns: (a) kinetochore-associated proteins (Sin3A, PCAF, MYST and BAF180), which colocalize with metaphase kinetochores, but not any of the pericentric and other major heterochromatic regions; and (b) heterochromatin-associated proteins (MeCP2, MBD1, MBD2, ATRX, HP1alpha, HDAC1, HDAC2, DNMT1 and DNMT3b), which colocalize with centromeric/pericentric heterochromatin and all other major heterochromatic sites. A heterogeneous third group (c) consists of the origin recognition complex subunit ORC2 and the histone acetyltransferase P300/CBP, which associate generally with kinetochores in humans and centromeric/pericentric heterochromatin in mouse, with some minor differences in localization. These observations indicate an extensive sharing of protein components involved in chromatin modification at gene loci, centromeres and various chromosomal heterochromatic landmarks. The definition of distinct patterns of chromosomal distribution for these proteins provides a useful basis for the further investigation of the broad-ranging roles of these proteins.
Language eng
DOI 10.1093/hmg/ddg330
Field of Research 06 Biological Sciences
11 Medical And Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2003, Oxford University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30119196

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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