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Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing

Bhatta, Tarka Raj, Chamings, Anthony, Vibin, Jessy and Alexandersen, Soren 2019, Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing, Scientific reports, vol. 9, no. 1, doi: 10.1038/s41598-019-41045-z.

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Title Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing
Author(s) Bhatta, Tarka Raj
Chamings, AnthonyORCID iD for Chamings, Anthony orcid.org/0000-0002-7762-4757
Vibin, Jessy
Alexandersen, SorenORCID iD for Alexandersen, Soren orcid.org/0000-0002-5039-3178
Journal name Scientific reports
Volume number 9
Issue number 1
Article ID 4602
Total pages 10
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2019-03-14
ISSN 2045-2322
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
CAMPYLOBACTER-UPSALIENSIS
GENETIC-CHARACTERIZATION
DOGS
PREVALENCE
IDENTIFICATION
INFECTION
ALIGNMENT
TOOL
Summary Gastroenteritis in young animals is a clinical presentation with many infectious and non- infectious aetiologies. We used next generation sequencing (NGS) to investigate the possible infectious causes of gastroenteritis in puppies from a dog kennel in Victoria, Australia. The near complete genome of a canine astrovirus was obtained from pooled faecal samples, and was found to be 94.7% identical with a canine astrovirus detected in the United Kingdom in 2012. The phylogenetic analysis of the capsid gene found similarities to those of canine astroviruses identified in Italy in 2005 and in UK and Hungary in 2012, but distant from that of a canine astrovirus previously identified in Australia in 2012. Thus, different serotypes of canine astrovirus are likely circulating in Australia. The close relationship to European astroviruses also suggested that there had been recent movements of ancestor canine astroviruses between Australia and Europe. NGS also detected other infections in the puppies including several canine papillomaviruses and a canine parvovirus (vaccine strain) as well as a very low level of campylobacter. Canine astrovirus was the probable cause of diarrhoea in these puppies, with the possible involvement of campylobacter bacteria. NGS was effective as a non-targeted method to determine the likely infectious cause of gastroenteritis.
Language eng
DOI 10.1038/s41598-019-41045-z
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Authors
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30120257

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.