Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid

Jiang, Liqin, Garcia, Mariana B., Hammond, David, Dahanayake, Dinasha and Wildsoet, Christine F. 2019, Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid, Investigative ophthalmology and visual science, vol. 60, no. 4, pp. 1226-1233, doi: 10.1167/iovs.18-25365.

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Title Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid
Author(s) Jiang, Liqin
Garcia, Mariana B.
Hammond, DavidORCID iD for Hammond, David orcid.org/0000-0002-8093-1555
Dahanayake, Dinasha
Wildsoet, Christine F.
Journal name Investigative ophthalmology and visual science
Volume number 60
Issue number 4
Start page 1226
End page 1233
Total pages 8
Publisher Association for Research in Vision and Ophthalmology
Place of publication Rockville, Md.
Publication date 2019-03
ISSN 0146-0404
1552-5783
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Ophthalmology
guinea pigs
experimental myopia
choroid
emmetropization
sensitivity
FORM-DEPRIVATION MYOPIA
LENS-INDUCED MYOPIA
EYE GROWTH
OCULAR GROWTH
SPECTACLE LENSES
THICKNESS
SCLERA
COMPENSATION
PREVALENCE
Summary Purpose: To investigate differences in sensitivity to myopia-inducing stimuli of two strains of pigmented guinea pigs. Methods: Eleven-day-old animals (New Zealand [NZ], n = 24 and Elm Hill strains [EH], n = 26) wore either a +2 or -2 diopter (D) lens over one eye and a plano lens over the fellow eye for 5 days; other 10-day-old EH (n = 9) and 7-day-old NZ (n = 9) animals were monocularly form-deprived (FD) for 28 days. Choroidal thickness and axial length were measured using A-scan ultrasonography at baseline and after 1 and 5 days for optical defocus treatments, and at baseline and after 28 days for the FD treatment. Refractive errors were measured by retinoscopy. Choroids of untreated animals were also evaluated using spectral-domain optical coherence tomography. Results: One day of optical defocus induced bidirectional (optical sign-dependent) choroidal responses in EH animals only (P < 0.01). Similar responses were detected in NZ animals after 5 days (P < 0.01), with concordant spherical equivalent refraction changes (P < 0.01). Compared with NZ animals, EH animals developed minimal myopia with FD after 28 days (-4.58 ± 0.97 vs. -0.69 ± 0.75 D for NZ versus EH, P < 0.001). Yet, EH animals showed paradoxical choroidal thickening, 20 ± 9 vs. -8 ± 8 μm for EH versus NZ, P < 0.001. Untreated EH animals also had significantly thicker choroids than NZ animals (147 ± 19 vs. 132 ± 16 μm, P < 0.05), with well-defined layering. Conclusions: As previously reported in chicks, guinea pigs show strain-related differences in response to myopia-inducing stimuli. The finding of a thicker, multilayered choroid in the strain showing decreased sensitivity to FD is provocative, suggesting a possible protective role of the choroid.
Language eng
DOI 10.1167/iovs.18-25365
Field of Research 11 Medical and Health Sciences
06 Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30120377

Document type: Journal Article
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School of Medicine
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