Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid

Jiang, Liqin, Garcia, Mariana B., Hammond, David, Dahanayake, Dinasha and Wildsoet, Christine F. 2019, Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid, Investigative ophthalmology and visual science, vol. 60, no. 4, pp. 1226-1233, doi: 10.1167/iovs.18-25365.

Attached Files
Name Description MIMEType Size Downloads

Title Strain-dependent differences in sensitivity to myopia-Inducing stimuli in guinea pigs and role of choroid
Author(s) Jiang, Liqin
Garcia, Mariana B.
Hammond, DavidORCID iD for Hammond, David
Dahanayake, Dinasha
Wildsoet, Christine F.
Journal name Investigative ophthalmology and visual science
Volume number 60
Issue number 4
Start page 1226
End page 1233
Total pages 8
Publisher Association for Research in Vision and Ophthalmology
Place of publication Rockville, Md.
Publication date 2019-03
ISSN 0146-0404
Keyword(s) Science & Technology
Life Sciences & Biomedicine
guinea pigs
experimental myopia
Summary Purpose: To investigate differences in sensitivity to myopia-inducing stimuli of two strains of pigmented guinea pigs. Methods: Eleven-day-old animals (New Zealand [NZ], n = 24 and Elm Hill strains [EH], n = 26) wore either a +2 or -2 diopter (D) lens over one eye and a plano lens over the fellow eye for 5 days; other 10-day-old EH (n = 9) and 7-day-old NZ (n = 9) animals were monocularly form-deprived (FD) for 28 days. Choroidal thickness and axial length were measured using A-scan ultrasonography at baseline and after 1 and 5 days for optical defocus treatments, and at baseline and after 28 days for the FD treatment. Refractive errors were measured by retinoscopy. Choroids of untreated animals were also evaluated using spectral-domain optical coherence tomography. Results: One day of optical defocus induced bidirectional (optical sign-dependent) choroidal responses in EH animals only (P < 0.01). Similar responses were detected in NZ animals after 5 days (P < 0.01), with concordant spherical equivalent refraction changes (P < 0.01). Compared with NZ animals, EH animals developed minimal myopia with FD after 28 days (-4.58 ± 0.97 vs. -0.69 ± 0.75 D for NZ versus EH, P < 0.001). Yet, EH animals showed paradoxical choroidal thickening, 20 ± 9 vs. -8 ± 8 μm for EH versus NZ, P < 0.001. Untreated EH animals also had significantly thicker choroids than NZ animals (147 ± 19 vs. 132 ± 16 μm, P < 0.05), with well-defined layering. Conclusions: As previously reported in chicks, guinea pigs show strain-related differences in response to myopia-inducing stimuli. The finding of a thicker, multilayered choroid in the strain showing decreased sensitivity to FD is provocative, suggesting a possible protective role of the choroid.
Language eng
DOI 10.1167/iovs.18-25365
Field of Research 11 Medical and Health Sciences
06 Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Authors
Persistent URL

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Checking
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 36 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Thu, 04 Apr 2019, 12:05:47 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact