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Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling

Small, Lewin, Brandon, Amanda E., Parker, Benjamin L., Deshpande, Vinita, Samsudeen, Azrah F., Kowalski, Greg M., Reznick, Jane, Wilks, Donna L., Preston, Elaine, Bruce, Clinton R., James, David E., Turner, Nigel and Cooney, Gregory J. 2019, Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling, Molecular metabolism, vol. 25, pp. 107-118, doi: 10.1016/j.molmet.2019.04.006.

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Title Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling
Author(s) Small, Lewin
Brandon, Amanda E.
Parker, Benjamin L.
Deshpande, Vinita
Samsudeen, Azrah F.
Kowalski, Greg M.ORCID iD for Kowalski, Greg M. orcid.org/0000-0002-1599-017X
Reznick, Jane
Wilks, Donna L.
Preston, Elaine
Bruce, Clinton R.ORCID iD for Bruce, Clinton R. orcid.org/0000-0002-0515-3343
James, David E.
Turner, Nigel
Cooney, Gregory J.
Journal name Molecular metabolism
Volume number 25
Start page 107
End page 118
Total pages 12
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2019-07
ISSN 2212-8778
Keyword(s) Diurnal rhythms
Glucose uptake
Insulin action
Insulin signaling
Phosphoproteomics
Skeletal muscle
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
Summary OBJECTIVE: Energy metabolism and insulin action follow a diurnal rhythm. It is therefore important that investigations into dysregulation of these pathways are relevant to the physiology of this diurnal rhythm. METHODS: We examined glucose uptake, markers of insulin action, and the phosphorylation of insulin signaling intermediates in muscle of chow and high fat, high sucrose (HFHS) diet-fed rats over the normal diurnal cycle. RESULTS: HFHS animals displayed hyperinsulinemia but had reduced systemic glucose disposal and lower muscle glucose uptake during the feeding period. Analysis of gene expression, enzyme activity, protein abundance and phosphorylation revealed a clear diurnal regulation of substrate oxidation pathways with no difference in Akt signaling in muscle. Transfection of a constitutively active Akt2 into the muscle of HFHS rats did not rescue diet-induced reductions in insulin-stimulated glucose uptake. CONCLUSIONS: These studies suggest that reduced glucose uptake in muscle during the diurnal cycle induced by short-term HFHS-feeding is not the result of reduced insulin signaling.
Language eng
DOI 10.1016/j.molmet.2019.04.006
Indigenous content off
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Authors
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30121228

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.